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Open AccessArticle

Establishment and Characterization of Immortalized Miniature Pig Pancreatic Cell Lines Expressing Oncogenic K-RasG12D

1
Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Korea
2
Department of Biological Science, College of Natural Sciences, Wonkwang University, 460, Iksan-daero, Iksan-si 54538, Korea
3
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Korea
4
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, 501 Jinjudaero, Jinju 52828, Korea
5
Department of Functional Genomics, KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon 34113, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(22), 8820; https://doi.org/10.3390/ijms21228820
Received: 30 October 2020 / Revised: 17 November 2020 / Accepted: 19 November 2020 / Published: 21 November 2020
(This article belongs to the Special Issue Biological Interfaces in Gastrointestinal Cancer)
In recent decades, many studies on the treatment and prevention of pancreatic cancer have been conducted. However, pancreatic cancer remains incurable, with a high mortality rate. Although mouse models have been widely used for preclinical pancreatic cancer research, these models have many differences from humans. Therefore, large animals may be more useful for the investigation of pancreatic cancer. Pigs have recently emerged as a new model of pancreatic cancer due to their similarities to humans, but no pig pancreatic cancer cell lines have been established for use in drug screening or analysis of tumor biology. Here, we established and characterized an immortalized miniature pig pancreatic cell line derived from primary pancreatic cells and pancreatic cancer-like cells expressing K-rasG12D regulated by the human PTF1A promoter. Using this immortalized cell line, we analyzed the gene expression and phenotypes associated with cancer cell characteristics. Notably, we found that acinar-to-ductal transition was caused by K-rasG12D in the cell line constructed from acinar cells. This may constitute a good research model for the analysis of acinar-to-ductal metaplasia in human pancreatic cancer. View Full-Text
Keywords: pancreatic ductal adenocarcinoma; acinar-to-ductal metaplasia; K-rasG12D; miniature pig pancreatic cells pancreatic ductal adenocarcinoma; acinar-to-ductal metaplasia; K-rasG12D; miniature pig pancreatic cells
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Yang, H.-J.; Song, B.-S.; Sim, B.-W.; Jung, Y.; Chae, U.; Lee, D.G.; Cha, J.-J.; Baek, S.-J.; Lim, K.S.; Choi, W.S.; Lee, H.-Y.; Son, H.-C.; Park, S.-H.; Jeong, K.-J.; Kang, P.; Baek, S.H.; Koo, B.-S.; Kim, H.-N.; Jin, Y.B.; Park, Y.-H.; Choo, Y.-K.; Kim, S.-U. Establishment and Characterization of Immortalized Miniature Pig Pancreatic Cell Lines Expressing Oncogenic K-RasG12D. Int. J. Mol. Sci. 2020, 21, 8820.

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