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Article

A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response

1
Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic
2
Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech Republic
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Department of Environmental Analytical Chemistry, Institute of Analytical Chemistry, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech Republic
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Section of Animal Physiology and Immunology, Department of Experimental Biology, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic
5
Department of Chemistry, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8738; https://doi.org/10.3390/ijms21228738
Received: 22 October 2020 / Revised: 13 November 2020 / Accepted: 17 November 2020 / Published: 19 November 2020
(This article belongs to the Special Issue Heavy Metal Toxicity in Humans)
The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO3)2 NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover the effects of their exposure on individual target organs and to reveal potential variability in the lead clearance. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs. In the lungs and liver, Pb(NO3)2 NP inhalation induced serious structural changes and their damage was present even after a 5-week clearance period despite the lead having been almost completely eliminated from the tissues. The numbers of macrophages significantly decreased after 11-week Pb(NO3)2 NP inhalation; conversely, abundance of alpha-smooth muscle actin (α-SMA)-positive cells, which are responsible for augmented collagen production, increased in both tissues. Moreover, the expression of nuclear factor κB (NF-κB) and selected cytokines, such as tumor necrosis factor alpha (TNFα), transforming growth factor beta 1 (TGFβ1), interleukin 6(IL-6), IL-1α and IL-1β , displayed a tissue-specific response to lead exposure. In summary, diminished inflammatory response in tissues after Pb(NO3)2 NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by sustained pathological changes in target organs, even after long clearance period. View Full-Text
Keywords: clearance; lead nanoparticles; inhalation; LA-ICP-MS imaging; toxicity clearance; lead nanoparticles; inhalation; LA-ICP-MS imaging; toxicity
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MDPI and ACS Style

Dumková, J.; Smutná, T.; Vrlíková, L.; Dočekal, B.; Kristeková, D.; Večeřa, Z.; Husáková, Z.; Jakešová, V.; Jedličková, A.; Mikuška, P.; Alexa, L.; Coufalík, P.; Tvrdoňová, M.; Křůmal, K.; Vaculovič, T.; Kanický, V.; Hampl, A.; Buchtová, M. A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response. Int. J. Mol. Sci. 2020, 21, 8738. https://doi.org/10.3390/ijms21228738

AMA Style

Dumková J, Smutná T, Vrlíková L, Dočekal B, Kristeková D, Večeřa Z, Husáková Z, Jakešová V, Jedličková A, Mikuška P, Alexa L, Coufalík P, Tvrdoňová M, Křůmal K, Vaculovič T, Kanický V, Hampl A, Buchtová M. A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response. International Journal of Molecular Sciences. 2020; 21(22):8738. https://doi.org/10.3390/ijms21228738

Chicago/Turabian Style

Dumková, Jana, Tereza Smutná, Lucie Vrlíková, Bohumil Dočekal, Daniela Kristeková, Zbyněk Večeřa, Zuzana Husáková, Veronika Jakešová, Adriena Jedličková, Pavel Mikuška, Lukáš Alexa, Pavel Coufalík, Michaela Tvrdoňová, Kamil Křůmal, Tomáš Vaculovič, Viktor Kanický, Aleš Hampl, and Marcela Buchtová. 2020. "A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response" International Journal of Molecular Sciences 21, no. 22: 8738. https://doi.org/10.3390/ijms21228738

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