Effects of Transient Receptor Potential Cation 5 (TRPC5) Inhibitor, NU6027, on Hippocampal Neuronal Death after Traumatic Brain Injury
Abstract
:1. Introduction
2. Results
2.1. TRPC5 Inhibitor Reduces TBI-Induced Hippocampal Neuronal Death
2.2. TRPC5 Inhibitor Reduces TBI-Induced Zinc Accumulation
2.3. TRPC5 Inhibitor Decreases TBI-Induced Hippocampal Dendrite Loss
2.4. TRPC5 Inhibitor Decreased TBI-Induced Oxidative Injury
2.5. TRPC5 Inhibitor Reduces TBI-Induced Hippocampal TRPC5 Channel Expression
2.6. TRPC5 Inhibitor Reduces TBI-Induced Hippocampal Glial Activation
2.7. TRPC5 Inhibitor Reduces TBI-Induced Hippocampal Neuron Loss
2.8. TRPC5 Inhibitor Reduces TBI-Induced Cognitive Impairment
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Experimental Animals
4.3. Controlled Cortical Impact Model for TBI
4.4. NU6027 Administration
4.5. Brain Sample Preparation
4.6. Confirmation of Hippocampal Neuron Degeneration
4.7. Confirmation of Hippocampal Zinc Accumulation
4.8. Confirmation of Hippocampal Microtubule Loss
4.9. Confirmation of Hippocampal Oxidative Stress
4.10. Confirmation of Hippocampal TRCP5 Channel Expression Level
4.11. Confirmation of Hippocampal Glial Activation
4.12. Confirmation of Hippocampal Live Neuron
4.13. Confirmation of Behavior Test
4.14. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Park, M.K.; Choi, B.Y.; Kho, A.R.; Lee, S.H.; Hong, D.K.; Jeong, J.H.; Kang, D.H.; Kang, B.S.; Suh, S.W. Effects of Transient Receptor Potential Cation 5 (TRPC5) Inhibitor, NU6027, on Hippocampal Neuronal Death after Traumatic Brain Injury. Int. J. Mol. Sci. 2020, 21, 8256. https://doi.org/10.3390/ijms21218256
Park MK, Choi BY, Kho AR, Lee SH, Hong DK, Jeong JH, Kang DH, Kang BS, Suh SW. Effects of Transient Receptor Potential Cation 5 (TRPC5) Inhibitor, NU6027, on Hippocampal Neuronal Death after Traumatic Brain Injury. International Journal of Molecular Sciences. 2020; 21(21):8256. https://doi.org/10.3390/ijms21218256
Chicago/Turabian StylePark, Min Kyu, Bo Young Choi, A Ra Kho, Song Hee Lee, Dae Ki Hong, Jeong Hyun Jeong, Dong Hyeon Kang, Beom Seok Kang, and Sang Won Suh. 2020. "Effects of Transient Receptor Potential Cation 5 (TRPC5) Inhibitor, NU6027, on Hippocampal Neuronal Death after Traumatic Brain Injury" International Journal of Molecular Sciences 21, no. 21: 8256. https://doi.org/10.3390/ijms21218256