Next Article in Journal
Genotype-Based Gene Expression in Colon Tissue—Prediction Accuracy and Relationship with the Prognosis of Colorectal Cancer Patients
Next Article in Special Issue
Nanoliposomes and Nanoemulsions Based on Chia Seed Lipids: Preparation and Characterization
Previous Article in Journal
Utilizing PacBio Iso-Seq for Novel Transcript and Gene Discovery of Abiotic Stress Responses in Oryza sativa L.
Previous Article in Special Issue
Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization
Article

Fluorescence Correlation Spectroscopy Reveals Interaction of Some Microdomain-Associated Lipids with Cellular Focal Adhesion Sites

1
Institute of Biological Information Processing, IBI-2: Mechanobiology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany
2
Experimental Medical Physics, Department of Physics, Heinrich-Heine Universität Düsseldorf, 40225 Düsseldorf, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8149; https://doi.org/10.3390/ijms21218149
Received: 21 August 2020 / Revised: 27 October 2020 / Accepted: 29 October 2020 / Published: 31 October 2020
(This article belongs to the Special Issue Functionalized Liposomes)
Cells adhere to the extracellular matrix at distinct anchoring points, mostly focal adhesions. These are rich in immobile transmembrane- and cytoskeletal-associated proteins, some of which are known to interact with lipids of the plasma membrane. To investigate their effect on lipid mobility and molecular interactions, fluorescently labeled lipids were incorporated into the plasma membranes of primary myofibroblasts using fusogenic liposomes. With fluorescence correlation spectroscopy, we tested mobilities of labeled microdomain-associated lipids such as sphingomyelin (SM), ganglioside (GM1), and cholesterol as well as of a microdomain-excluded phospholipid (PC) and a lipid-like molecule (DiIC18(7)) in focal adhesions (FAs) and in neighboring non-adherent membrane areas. We found significantly slower diffusion of SM and GM1 inside FAs but no effect on cholesterol, PC, and DiIC18(7). These data were compared to the molecular behavior in Lo/Ld-phase separated giant unilamellar vesicles, which served as a model system for microdomain containing lipid membranes. In contrast to the model system, lipid mobility changes in FAs were molecularly selective, and no particle enrichment occurred. Our findings suggest that lipid behavior in FAs cannot be described by Lo/Ld-phase separation. The observed slow-down of some molecules in FAs is potentially due to transient binding between lipids and some molecular constituent(s). View Full-Text
Keywords: lipid diffusion; focal adhesion site; fluorescence correlation spectroscopy (FCS); membrane microdomains; model membrane; liquid ordered and disordered phases; fusogenic liposomes lipid diffusion; focal adhesion site; fluorescence correlation spectroscopy (FCS); membrane microdomains; model membrane; liquid ordered and disordered phases; fusogenic liposomes
Show Figures

Graphical abstract

MDPI and ACS Style

Kleusch, C.; Monzel, C.; Sridhar, K.C.; Hoffmann, B.; Csiszár, A.; Merkel, R. Fluorescence Correlation Spectroscopy Reveals Interaction of Some Microdomain-Associated Lipids with Cellular Focal Adhesion Sites. Int. J. Mol. Sci. 2020, 21, 8149. https://doi.org/10.3390/ijms21218149

AMA Style

Kleusch C, Monzel C, Sridhar KC, Hoffmann B, Csiszár A, Merkel R. Fluorescence Correlation Spectroscopy Reveals Interaction of Some Microdomain-Associated Lipids with Cellular Focal Adhesion Sites. International Journal of Molecular Sciences. 2020; 21(21):8149. https://doi.org/10.3390/ijms21218149

Chicago/Turabian Style

Kleusch, Christian, Cornelia Monzel, Krishna C. Sridhar, Bernd Hoffmann, Agnes Csiszár, and Rudolf Merkel. 2020. "Fluorescence Correlation Spectroscopy Reveals Interaction of Some Microdomain-Associated Lipids with Cellular Focal Adhesion Sites" International Journal of Molecular Sciences 21, no. 21: 8149. https://doi.org/10.3390/ijms21218149

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop