Genotype-Based Gene Expression in Colon Tissue—Prediction Accuracy and Relationship with the Prognosis of Colorectal Cancer Patients
Abstract
:1. Introduction
2. Results
2.1. Correlation between Measured and Genetically Predicted Gene Expression
2.2. Association of Genetically Predicted Gene Expression and Colorectal Cancer Patients’ Survival
3. Discussion
4. Materials and Methods
4.1. Study Population
4.2. Genotyping
4.3. Gene Expression Measurement
4.4. Gene Expression Prediction
4.5. Statistical Analysis
4.6. Validation Set
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Overall Survival | CRC-Specific Survival | |||||||
---|---|---|---|---|---|---|---|---|
Variable | Level | Patients | Deaths | HR 1 (95% CI) | p | Deaths | HR 1 (95% CI) | p |
Age at | <60 | 946 | 257 | Ref. | <2 × 10−16 | 209 | Ref. | 0.0003 |
Diagnosis | 60–69 | 1339 | 466 | 1.25 (1.07−1.46) 1.69 (1.47−1.96) 3.20 (2.74−3.74) | 314 | 1.06 (0.89−1.27) 1.12 (0.96−1.36) 1.47 (1.20−1.80) | ||
(years) | 70–79 | 1489 | 638 | 357 | ||||
>80 | 662 | 429 | 173 | |||||
Gender | Male | 2685 | 1111 | Ref. | 0.16 | 626 | Ref. | 0.47 |
Female | 1751 | 679 | 1.07 (0.97−1.18) | 427 | 0.96 (0.85−1.08) | |||
CRC stage | I | 1024 | 235 | Ref. | <2 × 10−16 | 40 | Ref. | <2 × 10−16 |
II | 1345 | 434 | 1.49 (1.27−1.74) 2.05 (1.76−2.39) 10.21 (8.73−11.94) | 148 | 2.96 (2.09−4.20) 7.29 (5.25−10.10) 48.91 (35.37−67.64) | |||
III | 1426 | 575 | 354 | |||||
IV | 641 | 546 | 511 | |||||
Tumor site | Colon | 2665 | 1069 | Ref. | 0.61 | 598 | Ref. | 0.03 |
Rectum | 1771 | 721 | 1.03 (0.93–1.13) | 455 | 1.15 (1.02−1.30) | |||
Body mass | <18.5 | 121 | 71 | 1.74 (1.37−2.23) | 0.0006 | 41 | 1.62 (1.17−2.23) | 0.01 |
index | 18.5−24.9 | 1592 | 675 | Ref. | 396 | Ref. | ||
(kg/m2) | 25–29.9 | 1871 | 726 | 0.87 (0.78−0.96) 0.83 (0.73−0.95) | 432 | 0.88 (0.77−1.01) 0.83 (0.70–0.99) | ||
≥30 | 852 | 318 | 184 | |||||
Diabetes | No | 3594 | 1360 | Ref. | <8 × 10−13 | 844 | Ref. | 0.09 |
Yes | 811 | 412 | 1.50 (1.34−1.67) | 200 | 1.14 (0.98−1.33) | |||
Regular | No | 3272 | 1298 | Ref. | 0.05 | 793 | Ref. | 0.31 |
NSAID use | Yes | 1105 | 469 | 1.11 (1.00−1.24) | 245 | 0.93 (0.80−1.07) | ||
Smoking | Never | 1921 | 840 | Ref. | 0.04 | 515 | Ref. | 0.07 |
Former | 1769 | 684 | 0.90 (0.81−1.00) 0.89 (0.78−1.03) | 367 | 0.79 (0.69−0.90) 0.94 (0.79−1.12) | |||
Current | 672 | 263 | 169 | |||||
Alcohol | No intake | 1320 | 606 | Ref. | 0.0003 | 339 | Ref. | 0.01 |
intake | 0.1−5.6 | 856 | 318 | 0.75 (0.66−0.86) 0.72 (0.63−0.83) 0.78 (0.68−0.90) 0.79 (0.69−0.92) | 202 | 0.87 (0.73−1.03) 1.22 (0.68−0.98) 1.20 (0.70−1.00) 1.24 (0.66−0.97) | ||
(g/day) | 5.7−13.2 | 770 | 280 | 175 | ||||
13.3−28.5 | 775 | 301 | 177 | |||||
≥28.6 | 669 | 269 | 148 |
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Deutelmoser, H.; Lorenzo Bermejo, J.; Benner, A.; Weigl, K.; Park, H.A.; Haffa, M.; Herpel, E.; Schneider, M.; Ulrich, C.M.; Hoffmeister, M.; et al. Genotype-Based Gene Expression in Colon Tissue—Prediction Accuracy and Relationship with the Prognosis of Colorectal Cancer Patients. Int. J. Mol. Sci. 2020, 21, 8150. https://doi.org/10.3390/ijms21218150
Deutelmoser H, Lorenzo Bermejo J, Benner A, Weigl K, Park HA, Haffa M, Herpel E, Schneider M, Ulrich CM, Hoffmeister M, et al. Genotype-Based Gene Expression in Colon Tissue—Prediction Accuracy and Relationship with the Prognosis of Colorectal Cancer Patients. International Journal of Molecular Sciences. 2020; 21(21):8150. https://doi.org/10.3390/ijms21218150
Chicago/Turabian StyleDeutelmoser, Heike, Justo Lorenzo Bermejo, Axel Benner, Korbinian Weigl, Hanla A. Park, Mariam Haffa, Esther Herpel, Martin Schneider, Cornelia M. Ulrich, Michael Hoffmeister, and et al. 2020. "Genotype-Based Gene Expression in Colon Tissue—Prediction Accuracy and Relationship with the Prognosis of Colorectal Cancer Patients" International Journal of Molecular Sciences 21, no. 21: 8150. https://doi.org/10.3390/ijms21218150