Vasectomy and Photoperiodic Regimen Modify the Protein Profile, Hormonal Content and Antioxidant Enzymes Activity of Ram Seminal Plasma
Abstract
:1. Introduction
2. Results
2.1. Vasectomy Modifies the Protein Profile of Ram Seminal Plasma
2.2. Vasectomy Decreases Melatonin and Testosterone Concentrations, but Not Antioxidant Enzyme Activity in Ram Seminal Plasma
3. Discussion
4. Materials and Methods
4.1. Animals and Seminal Plasma Extraction
4.1.1. Rams in the Mediterranean Climate
4.1.2. Rams under Equatorial Photoperiod
4.2. Seminal Plasma Protein Analyses
4.3. Seminal Plasma Hormonal Analyses
4.3.1. Melatonin Evaluation
4.3.2. Testosterone Evaluation
4.4. Seminal Plasma Antioxidant Enzymes Activity
4.4.1. Glutathione Peroxidase (GPx)
4.4.2. Glutathione Reductase (GRD)
4.4.3. Catalase
4.5. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
SP | Seminal plasma |
GPx | Glutathione peroxidase |
GRD | Glutathione reductase |
SOD | Superoxide dismutase |
DIGE | Difference gel electrophoresis |
Appendix A
Intact | Vasectomized | |||
---|---|---|---|---|
Creole | Romney Marsh | Creole | Romney Marsh | |
Total protein (mg/mL) | 36.51 ± 2.30 | 42.69 ± 2.18 | 52.05 ± 2.98 | 55.01 ± 10.12 |
Melatonin (pg/mL) | 32.78 ± 3.38 | 30.15 ± 1.50 | 46.63 ± 3.05 | 42.51 ± 4.52 |
Testosterone (ng/mL) | 3.06 ± 0.14 | 2.89 ± 0.18 | 1.92 ± 0.13 | 2.05 ± 0.14 |
Glutathione peroxidase (nmole/min mL) | 10.44 ± 0.82 | 10.15 ± 0.79 | 12.68 ± 1.43 | 9.58 ± 0.71 |
Glutathione reductase (nmole/min mL) | 2.41 ± 0.46 | 2.73 ± 0.42 | 4.50 ± 0.43 | 3.60 ± 0.29 |
Catalase (µmole/min mL) | 68.84 ± 16.00 | 91.21 ± 19.50 | 104.04 ± 20.06 | 93.75 ± 17.71 |
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Band Intensity × 103 (Arbitrary Units) | ||||
---|---|---|---|---|
Total Protein (mg/mL) | High MW Bands (250–75 kDa) | Medium MW Bands (75–37 kDa) | Low MW Bands (37–10 kDa) | |
Intact seasonal rams | 34.20 ± 5.42 a | 3.90 ± 1.43 a | 11.25 ± 3.54 | 9.95 ± 2.80 |
Vasectomized seasonal rams | 64.63 ± 6.36 b | 0.44 ± 0.08 b | 7.57 ± 1.0 | 11.80 ± 1.46 |
Intact non-seasonal rams | 39.60 ± 1.66 a | 3.54 ± 0.53 a | 9.45 ± 1.21 | 12.20 ± 1.47 |
Vasectomized non-seasonal rams | 53.40 ± 2.10 b | 0.60 ± 0.13 b | 10.56 ± 5.29 | 10.85 ± 3.44 |
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Carvajal-Serna, M.; Fatnassi, M.; Torres-Ruda, F.; Cardozo, J.A.; Grajales-Lombana, H.; Hammadi, M.; Abecia, J.A.; Muiño-Blanco, T.; Pérez-Pe, R.; Cebrián-Pérez, J.Á.; et al. Vasectomy and Photoperiodic Regimen Modify the Protein Profile, Hormonal Content and Antioxidant Enzymes Activity of Ram Seminal Plasma. Int. J. Mol. Sci. 2020, 21, 8063. https://doi.org/10.3390/ijms21218063
Carvajal-Serna M, Fatnassi M, Torres-Ruda F, Cardozo JA, Grajales-Lombana H, Hammadi M, Abecia JA, Muiño-Blanco T, Pérez-Pe R, Cebrián-Pérez JÁ, et al. Vasectomy and Photoperiodic Regimen Modify the Protein Profile, Hormonal Content and Antioxidant Enzymes Activity of Ram Seminal Plasma. International Journal of Molecular Sciences. 2020; 21(21):8063. https://doi.org/10.3390/ijms21218063
Chicago/Turabian StyleCarvajal-Serna, Melissa, Meriem Fatnassi, Felipe Torres-Ruda, Jaime Antonio Cardozo, Henry Grajales-Lombana, Mohamed Hammadi, Jose Alfonso Abecia, Teresa Muiño-Blanco, Rosaura Pérez-Pe, Jose Álvaro Cebrián-Pérez, and et al. 2020. "Vasectomy and Photoperiodic Regimen Modify the Protein Profile, Hormonal Content and Antioxidant Enzymes Activity of Ram Seminal Plasma" International Journal of Molecular Sciences 21, no. 21: 8063. https://doi.org/10.3390/ijms21218063