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Article

A Human Periodontal Ligament Fibroblast Cell Line as a New Model to Study Periodontal Stress

1
Department of Orthodontics and Orofacial Orthopedics, University Hospital of Erlangen, Friedrich-Alexander University Erlangen-Nuernberg, Glueckstr. 11, 91054 Erlangen, Germany
2
Department of Orthodontics, University Hospital Regensburg, Franz Josef Strauss Allee 11, 93053 Regensburg, Germany
3
Experimental Trauma Surgery, Department of Trauma Surgery, University Hospital Regensburg, Franz Josef Strauss Allee 11, 93053 Regensburg, Germany
4
Department of Medicine 3, Rheumatology and Immunology, University of Erlangen-Nuremberg, Glueckstr. 6, 91054 Erlangen, Germany
5
Institut für Biochemie, Emil-Fischer-Zentrum, Friedrich-Alexander-Universität Erlangen-Nürnberg, Fahrstr. 17, 91054 Erlangen, Germany
6
Department of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 7961; https://doi.org/10.3390/ijms21217961
Received: 29 September 2020 / Revised: 19 October 2020 / Accepted: 23 October 2020 / Published: 27 October 2020
The periodontal ligament (PDL) is exposed to different kinds of mechanical stresses such as bite force or orthodontic tooth movement. A simple and efficient model to study molecular responses to mechanical stress is the application of compressive force onto primary human periodontal ligament fibroblasts via glass disks. Yet, this model suffers from the need for primary cells from human donors which have a limited proliferative capacity. Here we show that an immortalized cell line, PDL-hTERT, derived from primary human periodontal ligament fibroblasts exhibits characteristic responses to glass disk-mediated compressive force resembling those of primary cells. These responses include induction and secretion of pro-inflammatory markers, changes in expression of extracellular matrix-reorganizing genes and induction of genes related to angiogenesis, osteoblastogenesis and osteoclastogenesis. The fact that PDL-hTERT cells can easily be transfected broadens their usefulness, as molecular gain- and loss-of-function studies become feasible. View Full-Text
Keywords: mechanical loading; orthodontic tooth movement; bone remodeling; cell culture techniques mechanical loading; orthodontic tooth movement; bone remodeling; cell culture techniques
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MDPI and ACS Style

Weider, M.; Schröder, A.; Docheva, D.; Rodrian, G.; Enderle, I.; Seidel, C.L.; Andreev, D.; Wegner, M.; Bozec, A.; Deschner, J.; Kirschneck, C.; Proff, P.; Gölz, L. A Human Periodontal Ligament Fibroblast Cell Line as a New Model to Study Periodontal Stress. Int. J. Mol. Sci. 2020, 21, 7961. https://doi.org/10.3390/ijms21217961

AMA Style

Weider M, Schröder A, Docheva D, Rodrian G, Enderle I, Seidel CL, Andreev D, Wegner M, Bozec A, Deschner J, Kirschneck C, Proff P, Gölz L. A Human Periodontal Ligament Fibroblast Cell Line as a New Model to Study Periodontal Stress. International Journal of Molecular Sciences. 2020; 21(21):7961. https://doi.org/10.3390/ijms21217961

Chicago/Turabian Style

Weider, Matthias, Agnes Schröder, Denitsa Docheva, Gabriele Rodrian, Isabel Enderle, Corinna L. Seidel, Darja Andreev, Michael Wegner, Aline Bozec, James Deschner, Christian Kirschneck, Peter Proff, and Lina Gölz. 2020. "A Human Periodontal Ligament Fibroblast Cell Line as a New Model to Study Periodontal Stress" International Journal of Molecular Sciences 21, no. 21: 7961. https://doi.org/10.3390/ijms21217961

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