Danger-Associated Peptide Regulates Root Growth by Promoting Protons Extrusion in an AHA2-Dependent Manner in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Pep1 Inhibits the Root Growth Dependent on pH Changes
2.2. Pep1-PEPR Promotes the Acidification of Apoplast in Root Apex
2.3. Pep1 Activates the PM H+-ATPase Activity to Regulate Root Growth
2.4. AHA2 Is Required to Regulate the Pep1 Signaling in Root Growth
2.5. PEPR2 Interacts with AHA2 In Vitro and In Vivo
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Peptides
4.3. Reverse Transcription PCR (RT-PCR) Analysis
4.4. Root Structure Analysis
4.5. Apoplast Acidification Analysis
4.6. Measurements of PM H+-ATPase Activity
4.7. Yeast Two-Hybrid Analysis
4.8. BiFC Assay
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Shen, N.; Jing, Y.; Tu, G.; Fu, A.; Lan, W. Danger-Associated Peptide Regulates Root Growth by Promoting Protons Extrusion in an AHA2-Dependent Manner in Arabidopsis. Int. J. Mol. Sci. 2020, 21, 7963. https://doi.org/10.3390/ijms21217963
Shen N, Jing Y, Tu G, Fu A, Lan W. Danger-Associated Peptide Regulates Root Growth by Promoting Protons Extrusion in an AHA2-Dependent Manner in Arabidopsis. International Journal of Molecular Sciences. 2020; 21(21):7963. https://doi.org/10.3390/ijms21217963
Chicago/Turabian StyleShen, Nuo, Yanping Jing, Guoqing Tu, Aigen Fu, and Wenzhi Lan. 2020. "Danger-Associated Peptide Regulates Root Growth by Promoting Protons Extrusion in an AHA2-Dependent Manner in Arabidopsis" International Journal of Molecular Sciences 21, no. 21: 7963. https://doi.org/10.3390/ijms21217963