Next Article in Journal
Placenta-Specific Genes, Their Regulation During Villous Trophoblast Differentiation and Dysregulation in Preterm Preeclampsia
Previous Article in Journal
Age-Associated TET2 Mutations: Common Drivers of Myeloid Dysfunction, Cancer and Cardiovascular Disease
Open AccessArticle

Isolation and Identification of a TaTDR-Like Wheat Gene Encoding a bHLH Domain Protein, Which Negatively Regulates Chlorophyll Biosynthesis in Arabidopsis

1
Key Laboratory of Crop Heterosis of Shaanxi Province, Wheat Breeding Engineering Research Center, Ministry of Education, College of Agronomy, Northwest A & F University, Yangling 712100, Shaanxi, China
2
College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(2), 629; https://doi.org/10.3390/ijms21020629
Received: 26 November 2019 / Revised: 12 January 2020 / Accepted: 15 January 2020 / Published: 17 January 2020
(This article belongs to the Section Molecular Plant Sciences)
Chlorophyll biosynthesis plays a vital role in chloroplast development and photosynthesis in plants. In this study, we identified an orthologue of the rice gene TDR (Oryza sativa L., Tapetum Degeneration Retardation) in wheat (Triticum aestivum L.) called TaTDR-Like (TaTDRL) by sequence comparison. TaTDRL encodes a putative 557 amino acid protein with a basic helix-loop-helix (bHLH) conserved domain at the C-terminal (295–344 aa). The TaTDRL protein localised to the nucleus and displayed transcriptional activation activity in a yeast hybrid system. TaTDRL was expressed in the leaf tissue and expression was induced by dark treatment. Here, we revealed the potential function of TaTDRL gene in wheat by utilizing transgenic Arabidopsis plants TaTDRL overexpressing (TaTDRL-OE) and TaTDRL-EAR (EAR-motif, a repression domain of only 12 amino acids). Compared with wild-type plants (WT), both TaTDRL-OE and TaTDRL-EAR were characterized by a deficiency of chlorophyll. Moreover, the expression level of the chlorophyll-related gene AtPORC (NADPH:protochlorophyllide oxidoreductase C) in TaTDRL-OE and TaTDRL-EAR was lower than that of WT. We found that TaTDRL physically interacts with wheat Phytochrome Interacting Factor 1 (PIF1) and Arabadopsis PIF1, suggesting that TaTDRL regulates light signaling during dark or light treatment. In summary, TaTDRL may respond to dark or light treatment and negatively regulate chlorophyll biosynthesis by interacting with AtPIF1 in transgenic Arabidopsis. View Full-Text
Keywords: bHLH transcription factor; chlorophyll biosynthesis; myc family; TaTDRL; wheat bHLH transcription factor; chlorophyll biosynthesis; myc family; TaTDRL; wheat
Show Figures

Figure 1

MDPI and ACS Style

Xia, Y.; Li, Z.; Wang, J.; Li, Y.; Ren, Y.; Du, J.; Song, Q.; Ma, S.; Song, Y.; Zhao, H.; Yang, Z.; Zhang, G.; Niu, N. Isolation and Identification of a TaTDR-Like Wheat Gene Encoding a bHLH Domain Protein, Which Negatively Regulates Chlorophyll Biosynthesis in Arabidopsis. Int. J. Mol. Sci. 2020, 21, 629. https://doi.org/10.3390/ijms21020629

AMA Style

Xia Y, Li Z, Wang J, Li Y, Ren Y, Du J, Song Q, Ma S, Song Y, Zhao H, Yang Z, Zhang G, Niu N. Isolation and Identification of a TaTDR-Like Wheat Gene Encoding a bHLH Domain Protein, Which Negatively Regulates Chlorophyll Biosynthesis in Arabidopsis. International Journal of Molecular Sciences. 2020; 21(2):629. https://doi.org/10.3390/ijms21020629

Chicago/Turabian Style

Xia, Yu; Li, Zheng; Wang, Junwei; Li, Yanhong; Ren, Yang; Du, Jingjing; Song, Qilu; Ma, Shoucai; Song, Yulong; Zhao, Huiyan; Yang, Zhiquan; Zhang, Gaisheng; Niu, Na. 2020. "Isolation and Identification of a TaTDR-Like Wheat Gene Encoding a bHLH Domain Protein, Which Negatively Regulates Chlorophyll Biosynthesis in Arabidopsis" Int. J. Mol. Sci. 21, no. 2: 629. https://doi.org/10.3390/ijms21020629

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop