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Open AccessArticle

Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410

by Yan Liu 1, Yanmin Qie 1,2, Xing Li 1,3, Meinan Wang 1 and Xianming Chen 1,4,*
1
Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA
2
Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, 162 Hengshan Street, Gaoxin District, Shijiazhuang, Hebei 050035, China
3
College of Plant Protection, Hebei Agricultural University, Baoding, Hebei 071001, China
4
US Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics, and Quality Research Unit, Pullman, WA 99164-6430, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(2), 478; https://doi.org/10.3390/ijms21020478
Received: 20 December 2019 / Revised: 8 January 2020 / Accepted: 9 January 2020 / Published: 12 January 2020
(This article belongs to the Collection Genetics and Molecular Breeding in Plants)
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Genetic resistance is the best strategy for control of the disease. Spring wheat landrace PI 181410 has shown high level resistance to stripe rust. The present study characterized the landrace to have both race-specific all-stage resistance and nonrace-specific high-temperature adult-plant (HTAP) resistance. To map quantitative trait loci (QTL) for the resistance in PI 181410, it was crossed with Avocet S (AvS), from which a recombinant inbred line population was developed. The F5–F8 populations were consecutively phenotyped for stripe rust response in multiple field environments under natural Pst infection, and the F7 population was phenotyped in seedlings at low temperature and in adult-plant stage with selected Pst races in the greenhouse. The F7 population was genotyped using the 90K wheat SNP chip. Three QTL, QYrPI181410.wgp-4AS, QYrPI181410.wgp-4BL, and QYrPI181410.wgp-5BL.1, from PI 181410 for all-stage resistance, were mapped on chromosome arms 4AS, 4BL, and 5BL, respectively. Four QTL, QYrPI181410.wgp-1BL, QYrPI181410.wgp-4BL, QYrPI181410.wgp-5AS, and QYrPI181410.wgp-5BL.2, were identified from PI 181410 for HTAP resistance and mapped to 1BL, 4BL, 5AS, and 5BL, respectively. Two QTL with minor effects on stripe rust response were identified from AvS and mapped to 2BS and 2BL. Four of the QTL from PI 181410 and one from AvS were potentially new. As the 4BL QTL was most effective and likely a new gene for stripe rust resistance, three kompetitive allele specific PCR (KASP) markers were developed for incorporating this gene into new wheat cultivars. View Full-Text
Keywords: disease resistance; Puccinia striiformis; QTL mapping; stripe rust; Triticum aestivum; wheat disease resistance; Puccinia striiformis; QTL mapping; stripe rust; Triticum aestivum; wheat
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MDPI and ACS Style

Liu, Y.; Qie, Y.; Li, X.; Wang, M.; Chen, X. Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410. Int. J. Mol. Sci. 2020, 21, 478.

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