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Article

Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

by
Roubi Abuobeid
1,
Luis Herrera-Marcos
1,2,
María A. Navarro
1,2,3,
Carmen Arnal
2,3,4,
Roberto Martínez-Beamonte
2,3,5,
Joaquín Surra
2,3,5 and
Jesús Osada
1,2,3,*
1
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza, E-50013 Zaragoza, Spain
2
Instituto Agroalimentario de Aragón, CITA-Universidad de Zaragoza, E-50013 Zaragoza, Spain
3
CIBER de Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, E-28029 Madrid, Spain
4
Departamento de Patología Animal, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza, E-50013 Zaragoza, Spain
5
Departamento de Producción Animal y Ciencia de los Alimentos, Escuela Politécnica de Huesca, Instituto de Investigación Sanitaria de Aragón-Universidad de Zaragoza, E-22071 Huesca, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 7331; https://doi.org/10.3390/ijms21197331
Submission received: 4 August 2020 / Revised: 21 September 2020 / Accepted: 29 September 2020 / Published: 4 October 2020
(This article belongs to the Special Issue Nutrition Genomics)
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Abstract

Erythrodiol is a terpenic compound found in a large number of plants. To test the hypotheses that its long-term administration may influence hepatic transcriptome and this could be influenced by the presence of APOA1-containing high-density lipoproteins (HDL), Western diets containing 0.01% of erythrodiol (10 mg/kg dose) were provided to Apoe- and Apoa1-deficient mice. Hepatic RNA-sequencing was carried out in male Apoe-deficient mice fed purified Western diets differing in the erythrodiol content. The administration of this compound significantly up- regulated 68 and down-regulated 124 genes at the level of 2-fold change. These genes belonged to detoxification processes, protein metabolism and nucleic acid related metabolites. Gene expression changes of 21 selected transcripts were verified by RT-qPCR. Ccl19-ps2, Cyp2b10, Rbm14-rbm4, Sec61g, Tmem81, Prtn3, Amy2a5, Cyp2b9 and Mup1 showed significant changes by erythrodiol administration. When Cyp2b10, Dmbt1, Cyp2b13, Prtn3 and Cyp2b9 were analyzed in female Apoe-deficient mice, no change was observed. Likewise, no significant variation was observed in Apoa1- or in Apoe-deficient mice receiving doses ranging from 0.5 to 5 mg/kg erythrodiol. Our results give evidence that erythrodiol exerts a hepatic transcriptional role, but this is selective in terms of sex and requires a threshold dose. Furthermore, it requires an APOA1-containing HDL.
Keywords: erythrodiol; mice; liver; apolipoprotein E; olive oil; transcriptome erythrodiol; mice; liver; apolipoprotein E; olive oil; transcriptome
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MDPI and ACS Style

Abuobeid, R.; Herrera-Marcos, L.; Navarro, M.A.; Arnal, C.; Martínez-Beamonte, R.; Surra, J.; Osada, J. Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way. Int. J. Mol. Sci. 2020, 21, 7331. https://doi.org/10.3390/ijms21197331

AMA Style

Abuobeid R, Herrera-Marcos L, Navarro MA, Arnal C, Martínez-Beamonte R, Surra J, Osada J. Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way. International Journal of Molecular Sciences. 2020; 21(19):7331. https://doi.org/10.3390/ijms21197331

Chicago/Turabian Style

Abuobeid, Roubi, Luis Herrera-Marcos, María A. Navarro, Carmen Arnal, Roberto Martínez-Beamonte, Joaquín Surra, and Jesús Osada. 2020. "Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way" International Journal of Molecular Sciences 21, no. 19: 7331. https://doi.org/10.3390/ijms21197331

APA Style

Abuobeid, R., Herrera-Marcos, L., Navarro, M. A., Arnal, C., Martínez-Beamonte, R., Surra, J., & Osada, J. (2020). Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way. International Journal of Molecular Sciences, 21(19), 7331. https://doi.org/10.3390/ijms21197331

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