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Open AccessArticle

The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients

1
Department of Molecular Biology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, Poland
2
Department of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, Poland
3
Department of Connective Tissue Diseases, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, Poland
4
Department of Pathophysiology, Warsaw Medical University, 02-091 Warsaw, Poland
5
Department of Rheumatology and Internal Medicine, Poznan University of Medical Science, 61-701 Poznan, Poland
6
Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, 61-701 Poznan, Poland
7
Department of Physiology, Pomeranian Medical University, 70-204 Szczecin, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 7169; https://doi.org/10.3390/ijms21197169
Received: 5 August 2020 / Revised: 25 September 2020 / Accepted: 25 September 2020 / Published: 28 September 2020
(This article belongs to the Special Issue Research of Pathogenesis and Novel Therapeutics in Arthritis 2.0)
MicroRNAs regulate gene expression of transcriptional factors, which influence Th17/Treg (regulatory T cells) balance, establishing the molecular mechanism of genetic and epigenetic regulation of Treg and Th17 cells is crucial for understanding rheumatoid arthritis (RA) pathogenesis. The study goal was to understand the potential impact of the selected microRNAs expression profiles on Treg/Th17 cells frequency, RA phenotype, the expression profile of selected microRNAs, and their correlation with the expression profiles of selected transcriptional factors: SOCS1, SMAD3, SMAD4, STAT3, STAT5 in RA; we used osteoarthritis (OA) and healthy controls (HCs) as controls. The study was conducted on 14 RA and 11 OA patients, and 15 HCs. Treg/Th17 frequency was established by flow cytometry. Gene expression analysis was estimated by qPCR. We noticed correlations in RA Th17 cells between miR-26 and SMAD3, STAT3, SOCS1; and miR-155 and STAT3—and in RA Treg cells between miR-26 and SOCS1; miR-31, -155 and SMAD3; and miR-155 and SMAD4. In RA Tregs, we found a negative correlation between miR-26, -126 and STAT5a. The expression level of miR-31 in Th17 cells from RA patients with DAS28 ≤ 5.1 is higher and that for miR-24 is greater in Tregs from patients with DAS28 > 5.1. MiR-146a in Tregs is higher in rheumatoid factor (RF) positive RA patients. View Full-Text
Keywords: rheumatoid arthritis (RA); Treg; Th17; gene expression; microRNA; transcriptional factor rheumatoid arthritis (RA); Treg; Th17; gene expression; microRNA; transcriptional factor
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MDPI and ACS Style

Kmiołek, T.; Rzeszotarska, E.; Wajda, A.; Walczuk, E.; Kuca-Warnawin, E.; Romanowska-Próchnicka, K.; Stypinska, B.; Majewski, D.; Jagodzinski, P.P.; Pawlik, A.; Paradowska-Gorycka, A. The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients. Int. J. Mol. Sci. 2020, 21, 7169. https://doi.org/10.3390/ijms21197169

AMA Style

Kmiołek T, Rzeszotarska E, Wajda A, Walczuk E, Kuca-Warnawin E, Romanowska-Próchnicka K, Stypinska B, Majewski D, Jagodzinski PP, Pawlik A, Paradowska-Gorycka A. The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients. International Journal of Molecular Sciences. 2020; 21(19):7169. https://doi.org/10.3390/ijms21197169

Chicago/Turabian Style

Kmiołek, Tomasz; Rzeszotarska, Ewa; Wajda, Anna; Walczuk, Ewa; Kuca-Warnawin, Ewa; Romanowska-Próchnicka, Katarzyna; Stypinska, Barbara; Majewski, Dominik; Jagodzinski, Pawel P.; Pawlik, Andrzej; Paradowska-Gorycka, Agnieszka. 2020. "The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients" Int. J. Mol. Sci. 21, no. 19: 7169. https://doi.org/10.3390/ijms21197169

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