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Article

Umbilical Cord Mesenchymal Stem Cell-Derived Nanovesicles Potentiate the Bone-Formation Efficacy of Bone Morphogenetic Protein 2

1
School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea
2
Department of Orthopedic Surgery, College of Medicine, SMG-SNU Boramae Medical Center, Seoul National University, Seoul 07061, Korea
3
Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, Seoul 07061, Korea
4
Institute of Chemical Processes, Institute of Engineering Research, BioMAX, Seoul National University, Seoul 08826, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(17), 6425; https://doi.org/10.3390/ijms21176425
Received: 31 July 2020 / Revised: 22 August 2020 / Accepted: 1 September 2020 / Published: 3 September 2020
(This article belongs to the Special Issue Bone Development and Regeneration)
Recombinant human bone morphogenetic protein 2 (rhBMP-2) is one of the most potent osteogenic factors used to treat bone loss. However, at higher doses, rhBMP-2 does not necessarily increase bone formation but rather increases the incidence of adverse side effects. Here, we investigated whether umbilical cord mesenchymal stem cell (UCMSC)-derived nanovesicles (NVs) further increase the in vivo bone formation at high doses of rhBMP-2. In the presence of UCMSC-derived NVs, proliferation, migration, and tube formation of human umbilical vein endothelial cells were stimulated in vitro. Furthermore, migration and osteogenesis of human bone marrow-derived mesenchymal stem cells were stimulated. To examine the efficacy of UCMSC-derived NVs on in vivo bone formation, collagen sponges soaked with rhBMP-2 and UCMSC-derived NVs were used in athymic nude mice with calvarial defects. At a high rhBMP-2 dosage (500 ng/mL), UCMSC-derived NVs significantly promoted bone formation in calvarial defects; however, the UCMSC-derived NVs alone did not induce in vivo bone formation. Our results indicate that UCMSC-derived NVs can potentiate the bone formation efficacy of rhBMP-2 at a high dosage. View Full-Text
Keywords: recombinant human bone morphogenetic protein 2; umbilical cord mesenchymal stem cell-derived nanovesicles; bone formation; angiogenesis; osteogenesis recombinant human bone morphogenetic protein 2; umbilical cord mesenchymal stem cell-derived nanovesicles; bone formation; angiogenesis; osteogenesis
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MDPI and ACS Style

Lim, S.; Lyu, H.-Z.; Lee, J.-R.; Han, S.H.; Lee, J.H.; Kim, B.-S. Umbilical Cord Mesenchymal Stem Cell-Derived Nanovesicles Potentiate the Bone-Formation Efficacy of Bone Morphogenetic Protein 2. Int. J. Mol. Sci. 2020, 21, 6425. https://doi.org/10.3390/ijms21176425

AMA Style

Lim S, Lyu H-Z, Lee J-R, Han SH, Lee JH, Kim B-S. Umbilical Cord Mesenchymal Stem Cell-Derived Nanovesicles Potentiate the Bone-Formation Efficacy of Bone Morphogenetic Protein 2. International Journal of Molecular Sciences. 2020; 21(17):6425. https://doi.org/10.3390/ijms21176425

Chicago/Turabian Style

Lim, Songhyun, Hao-Zhen Lyu, Ju-Ro Lee, Shi H. Han, Jae H. Lee, and Byung-Soo Kim. 2020. "Umbilical Cord Mesenchymal Stem Cell-Derived Nanovesicles Potentiate the Bone-Formation Efficacy of Bone Morphogenetic Protein 2" International Journal of Molecular Sciences 21, no. 17: 6425. https://doi.org/10.3390/ijms21176425

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