Next Article in Journal
Airway Inflammation and Host Responses in the Era of CFTR Modulators
Next Article in Special Issue
Modification of Serine 1040 of SIBRI1 Increases Fruit Yield by Enhancing Tolerance to Heat Stress in Tomato
Previous Article in Journal
Antimicrobial and Amyloidogenic Activity of Peptides Synthesized on the Basis of the Ribosomal S1 Protein from Thermus Thermophilus
Previous Article in Special Issue
Retrograde Signaling: Understanding the Communication between Organelles
Open AccessArticle

Cell Type-Specific Imaging of Calcium Signaling in Arabidopsis thaliana Seedling Roots Using GCaMP3

Noble Research Institute LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6385;
Received: 22 July 2020 / Revised: 27 August 2020 / Accepted: 28 August 2020 / Published: 2 September 2020
(This article belongs to the Special Issue Cell Signaling in Model Plants 2.0)
Cytoplasmic calcium ([Ca2+]cyt) is a well-characterized second messenger in eukaryotic cells. An elevation in [Ca2+]cyt levels is one of the earliest responses in plant cells after exposure to a range of environmental stimuli. Advances in understanding the role of [Ca2+]cyt in plant development has been facilitated by the use of genetically-encoded reporters such as GCaMP. Most of these studies have relied on promoters such as Cauliflower Mosaic Virus (35S) and Ubiquitin10 (UBQ10) to drive expression of GCaMP in all cell/tissue types. Plant organs such as roots consist of various cell types that likely exhibit unique [Ca2+]cyt responses to exogenous and endogenous signals. However, few studies have addressed this question. Here, we introduce a set of Arabidopsis thaliana lines expressing GCaMP3 in five root cell types including the columella, endodermis, cortex, epidermis, and trichoblasts. We found similarities and differences in the [Ca2+]cyt signature among these root cell types when exposed to adenosine tri-phosphate (ATP), glutamate, aluminum, and salt, which are known to trigger [Ca2+]cyt increases in root cells. These cell type-targeted GCaMP3 lines provide a new resource that should enable more in depth studies that address how a particular environmental stimulus is linked to specific root developmental pathways via [Ca2+]cyt. View Full-Text
Keywords: GCaMP3; Arabidopsis; cell type specificity; calcium response; [Ca2+]cyt GCaMP3; Arabidopsis; cell type specificity; calcium response; [Ca2+]cyt
Show Figures

Figure 1

MDPI and ACS Style

Krogman, W.; Sparks, J.A.; Blancaflor, E.B. Cell Type-Specific Imaging of Calcium Signaling in Arabidopsis thaliana Seedling Roots Using GCaMP3. Int. J. Mol. Sci. 2020, 21, 6385.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop