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Open AccessArticle

Cell Type-Specific Imaging of Calcium Signaling in Arabidopsis thaliana Seedling Roots Using GCaMP3

Noble Research Institute LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
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Int. J. Mol. Sci. 2020, 21(17), 6385; https://doi.org/10.3390/ijms21176385
Received: 22 July 2020 / Revised: 27 August 2020 / Accepted: 28 August 2020 / Published: 2 September 2020
(This article belongs to the Special Issue Cell Signaling in Model Plants 2.0)
Cytoplasmic calcium ([Ca2+]cyt) is a well-characterized second messenger in eukaryotic cells. An elevation in [Ca2+]cyt levels is one of the earliest responses in plant cells after exposure to a range of environmental stimuli. Advances in understanding the role of [Ca2+]cyt in plant development has been facilitated by the use of genetically-encoded reporters such as GCaMP. Most of these studies have relied on promoters such as Cauliflower Mosaic Virus (35S) and Ubiquitin10 (UBQ10) to drive expression of GCaMP in all cell/tissue types. Plant organs such as roots consist of various cell types that likely exhibit unique [Ca2+]cyt responses to exogenous and endogenous signals. However, few studies have addressed this question. Here, we introduce a set of Arabidopsis thaliana lines expressing GCaMP3 in five root cell types including the columella, endodermis, cortex, epidermis, and trichoblasts. We found similarities and differences in the [Ca2+]cyt signature among these root cell types when exposed to adenosine tri-phosphate (ATP), glutamate, aluminum, and salt, which are known to trigger [Ca2+]cyt increases in root cells. These cell type-targeted GCaMP3 lines provide a new resource that should enable more in depth studies that address how a particular environmental stimulus is linked to specific root developmental pathways via [Ca2+]cyt. View Full-Text
Keywords: GCaMP3; Arabidopsis; cell type specificity; calcium response; [Ca2+]cyt GCaMP3; Arabidopsis; cell type specificity; calcium response; [Ca2+]cyt
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Krogman, W.; Sparks, J.A.; Blancaflor, E.B. Cell Type-Specific Imaging of Calcium Signaling in Arabidopsis thaliana Seedling Roots Using GCaMP3. Int. J. Mol. Sci. 2020, 21, 6385.

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