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Open AccessArticle

In Vivo Optical Reporter-Gene-Based Imaging of Macrophage Infiltration of DNCB-Induced Atopic Dermatitis

by 1,†, 2,3,†, 2,4, 2,5 and 2,6,*
1
Korea Institute of Medical Microrobotics (KIMIRo), Gwangju 61011, Korea
2
Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 700-721, Korea
3
Research Center of Stickus Corporation, Haeundae-gu jaesong-dong 1050-21, Busan 48054, Korea
4
Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Pusan 50463, Korea
5
College of Veterinary Medicine, Kyungpook National University, Daegu 700-721, Korea
6
Leading-Edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University Hospital, Daegu 700-721, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(17), 6205; https://doi.org/10.3390/ijms21176205
Received: 18 August 2020 / Revised: 24 August 2020 / Accepted: 25 August 2020 / Published: 27 August 2020
(This article belongs to the Section Molecular Immunology)
This study was conducted to monitor the macrophage infiltration of atopic dermatitis (AD)-like skin lesions and to evaluate the effects of anti-AD therapeutic agents in immunocompetent mice via optical reporter-gene-based molecular imaging. The enhanced firefly luciferase (effluc)-expressing macrophage cell line (Raw264.7/effluc) was intravenously introduced into mice with 2,4-dinitrochlorobenzene (DNCB)-induced AD, followed by bioluminescent imaging (BLI). After in vivo imaging, AD-like skin lesions were excised, and ex vivo imaging and Western blotting were conducted to determine the presence of infused macrophages. Finally, the therapeutic effect of dexamethasone (DEX), an AD-modulating agent, was evaluated via macrophage tracking. In vivo imaging with BLI revealed the migration of the reporter macrophages to DNCB-induced AD-like skin lesions on day 1 post-transfer. The greatest recruitment was observed on day 3, and a decline in BLI signal was observed on day 14. Notably, in vivo BLI clearly showed the inhibition of the reporter macrophage infiltration of DNCB-induced AD-like skin lesions by DEX, which was consistent with the reduced AD symptoms observed in DEX-treated mice. We successfully visualized the macrophage migration to DNCB-induced AD-like skin lesions, proving the feasibility of macrophage imaging for evaluating AD-regulating drugs in living organisms. View Full-Text
Keywords: atopic dermatitis; reporter macrophages; enhanced firefly luciferase gene (effluc); cell tracking; bioluminescence imaging (BLI) atopic dermatitis; reporter macrophages; enhanced firefly luciferase gene (effluc); cell tracking; bioluminescence imaging (BLI)
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Lee, S.B.; Park, H.; Lee, J.-E.; Kim, K.-S.; Jeon, Y.H. In Vivo Optical Reporter-Gene-Based Imaging of Macrophage Infiltration of DNCB-Induced Atopic Dermatitis. Int. J. Mol. Sci. 2020, 21, 6205.

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