Next Article in Journal
Gestational Factors throughout Fetal Neurodevelopment: The Serotonin Link
Next Article in Special Issue
Oxidative Stress and Microglial Response in Retinitis Pigmentosa
Previous Article in Journal
Exosomes in Angiogenesis and Anti-angiogenic Therapy in Cancers
Previous Article in Special Issue
Effects of Hyperoxia on the Refraction in Murine Neonatal and Adult Models
Article

Hypothermically Stored Adipose-Derived Mesenchymal Stromal Cell Alginate Bandages Facilitate Use of Paracrine Molecules for Corneal Wound Healing

1
Biosciences Institute, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK
2
Atelerix Ltd., The Biosphere, Newcastle upon Tyne NE4 5BX, UK
3
UCL Institute of Ophthalmology, London EC1V 9EL, UK
4
Department of Ophthalmology, Royal Victoria Infirmary & Newcastle University, Newcastle upon Tyne NE1 4LP, UK
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(16), 5849; https://doi.org/10.3390/ijms21165849
Received: 2 July 2020 / Revised: 5 August 2020 / Accepted: 5 August 2020 / Published: 14 August 2020
(This article belongs to the Special Issue Molecular Research on Stress Response and Ocular Homeostasis)
Adipose-derived mesenchymal stromal cells (Ad-MSCs) may alleviate corneal injury through the secretion of therapeutic factors delivered at the injury site. We aimed to investigate the therapeutic factors secreted from hypothermically stored, alginate-encapsulated Ad-MSCs’ bandages in in vitro and in vivo corneal wounds. Ad-MSCs were encapsulated in 1.2% w/v alginate gels to form bandages and stored at 15 °C for 72 h before assessing cell viability and co-culture with corneal scratch wounds. Genes of interest, including HGF, TSG-6, and IGF were identified by qPCR and a human cytokine array kit used to profile the therapeutic factors secreted. In vivo, bandages were applied to adult male mice corneas following epithelial debridement. Bandages were shown to maintain Ad-MSCs viability during storage and able to indirectly improve corneal wound healing in vivo. Soluble protein concentration and paracrine factors such as TSG-6, HGF, IL-8, and MCP-1 release were greatest following hypothermic storage. In vivo, Ad-MSCs bandages-treated groups reduced immune cell infiltration when compared to untreated groups. In conclusion, bandages were shown to maintain Ad-MSCs ability to produce a cocktail of key therapeutic factors following storage and that these soluble factors can improve in vitro and in vivo corneal wound healing. View Full-Text
Keywords: cornea; stem cells bandage; paracrine factors; adipose-derived stem cells; hypothermic storage; sodium alginate; wound model cornea; stem cells bandage; paracrine factors; adipose-derived stem cells; hypothermic storage; sodium alginate; wound model
Show Figures

Figure 1

MDPI and ACS Style

Al-Jaibaji, O.; Swioklo, S.; Shortt, A.; Figueiredo, F.C.; Connon, C.J. Hypothermically Stored Adipose-Derived Mesenchymal Stromal Cell Alginate Bandages Facilitate Use of Paracrine Molecules for Corneal Wound Healing. Int. J. Mol. Sci. 2020, 21, 5849. https://doi.org/10.3390/ijms21165849

AMA Style

Al-Jaibaji O, Swioklo S, Shortt A, Figueiredo FC, Connon CJ. Hypothermically Stored Adipose-Derived Mesenchymal Stromal Cell Alginate Bandages Facilitate Use of Paracrine Molecules for Corneal Wound Healing. International Journal of Molecular Sciences. 2020; 21(16):5849. https://doi.org/10.3390/ijms21165849

Chicago/Turabian Style

Al-Jaibaji, Olla, Stephen Swioklo, Alex Shortt, Francisco C. Figueiredo, and Che J. Connon 2020. "Hypothermically Stored Adipose-Derived Mesenchymal Stromal Cell Alginate Bandages Facilitate Use of Paracrine Molecules for Corneal Wound Healing" International Journal of Molecular Sciences 21, no. 16: 5849. https://doi.org/10.3390/ijms21165849

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop