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Article

Proteome-Wide Analyses Provide New Insights into the Compatible Interaction of Rice with the Root-Knot Nematode Meloidogyne graminicola

1
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
3
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China
*
Authors to whom correspondence should be addressed.
These authors equally contributed to this work.
Int. J. Mol. Sci. 2020, 21(16), 5640; https://doi.org/10.3390/ijms21165640
Received: 20 July 2020 / Revised: 4 August 2020 / Accepted: 4 August 2020 / Published: 6 August 2020
(This article belongs to the Special Issue Plant Proteomic Research 3.0)
The root-knot nematode Meloidogyne graminicola is an important pathogen in rice, causing huge yield losses annually worldwide. Details of the interaction between rice and M. graminicola and the resistance genes in rice still remain unclear. In this study, proteome-wide analyses of the compatible interaction of the japonica rice cultivar “Nipponbare” (NPB) with M. graminicola were performed. In total, 6072 proteins were identified in NPB roots with and without infection of M. graminicola by label-free quantitative mass spectrometry. Of these, 513 specifically or significantly differentially expressed proteins were identified to be uniquely caused by nematode infection. Among these unique proteins, 99 proteins were enriched on seven Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. By comparison of protein expression and gene transcription, LOC_Os01g06600 (ACX, a glutaryl-CoA dehydrogenase), LOC_Os09g23560 (CAD, a cinnamyl-alcohol dehydrogenase), LOC_Os03g39850 (GST, a glutathione S-transferase) and LOC_Os11g11960 (RPM1, a disease resistance protein) on the alpha-linolenic acid metabolism, phenylpropanoid biosynthesis, glutathione metabolism and plant–pathogen interaction pathways, respectively, were all associated with disease defense and identified to be significantly down-regulated in the compatible interaction of NPB with nematodes, while the corresponding genes were remarkably up-regulated in the roots of a resistant rice accession “Khao Pahk Maw” with infection of nematodes. These four genes likely played important roles in the compatible interaction of rice with M. graminicola. Conversely, these disease defense-related genes were hypothesized to be likely involved in the resistance of resistant rice lines to this nematode. The proteome-wide analyses provided many new insights into the interaction of rice with M. graminicola. View Full-Text
Keywords: rice; root-knot nematode; Meloidogyne graminicola; compatible interaction; proteome-wide analyses rice; root-knot nematode; Meloidogyne graminicola; compatible interaction; proteome-wide analyses
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MDPI and ACS Style

Xiang, C.; Yang, X.; Peng, D.; Kang, H.; Liu, M.; Li, W.; Huang, W.; Liu, S. Proteome-Wide Analyses Provide New Insights into the Compatible Interaction of Rice with the Root-Knot Nematode Meloidogyne graminicola. Int. J. Mol. Sci. 2020, 21, 5640. https://doi.org/10.3390/ijms21165640

AMA Style

Xiang C, Yang X, Peng D, Kang H, Liu M, Li W, Huang W, Liu S. Proteome-Wide Analyses Provide New Insights into the Compatible Interaction of Rice with the Root-Knot Nematode Meloidogyne graminicola. International Journal of Molecular Sciences. 2020; 21(16):5640. https://doi.org/10.3390/ijms21165640

Chicago/Turabian Style

Xiang, Chao, Xiaoping Yang, Deliang Peng, Houxiang Kang, Maoyan Liu, Wei Li, Wenkun Huang, and Shiming Liu. 2020. "Proteome-Wide Analyses Provide New Insights into the Compatible Interaction of Rice with the Root-Knot Nematode Meloidogyne graminicola" International Journal of Molecular Sciences 21, no. 16: 5640. https://doi.org/10.3390/ijms21165640

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