Next Article in Journal
Stress Granule Formation Attenuates RACK1-Mediated Apoptotic Cell Death Induced by Morusin
Next Article in Special Issue
The Role of the Epididymis and the Contribution of Epididymosomes to Mammalian Reproduction
Previous Article in Journal
Evaluation of Mussel Shells Powder as Reinforcement for PLA-Based Biocomposites
Previous Article in Special Issue
Oviduct Fluid Extracellular Vesicles Change the Phospholipid Composition of Bovine Embryos Developed In Vitro
Article

Bovine Follicular Fluid and Extracellular Vesicles Derived from Follicular Fluid Alter the Bovine Oviductal Epithelial Cells Transcriptome

1
Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila St. 14b, 50411 Tartu, Estonia
2
Competence Centre on Health Technologies, Tiigi St. 61b, 50410 Tartu, Estonia
3
Department of Chemistry and Biotechnology, School of Science, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia
4
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima St. 10, 10-748 Olsztyn, Poland
5
Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006 Tartu, Estonia
6
Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, L. Puusepa St. 8, 50406 Tartu, Estonia
7
Institute of Genomics, University of Tartu, Riia St. 23b, 51010 Tartu, Estonia
8
Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, The Medical School, University of Sheffield, Sheffield S10 2SF, UK
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(15), 5365; https://doi.org/10.3390/ijms21155365
Received: 16 May 2020 / Revised: 21 July 2020 / Accepted: 22 July 2020 / Published: 28 July 2020
(This article belongs to the Special Issue Extracellular Vesicles in Reproduction)
While follicular fluid (FF) is well known to provide an optimal environment for oogenesis, its functional roles following its release into the oviduct during ovulation are currently elusive. We hypothesized that FF and FF-derived extracellular vesicles (EVs) may be conveyors of signals capable of inducing functionally-relevant transcriptional responses in oviductal cells. The aim of this study was, therefore, to evaluate the effect of FF and FF-derived EVs on the transcriptome of primary bovine oviductal epithelial cells (BOECs). We examined the gene expression of BOECs in three conditions: BOECs cultured with FF, FF-derived EVs, and without supplementations. For each condition, cells were cultured for 6 and 24 h. RNA sequencing results revealed that FF had a stronger effect on BOECs gene expression compared to EVs. We detected 488 and 1998 differentially expressed genes (DEGs) with FF treatment in 6 and 24 h, respectively, whereas only 41 DEGs were detected at 6 h following EV treatment. Pathway analysis of the FF-induced DEGs showed that several pathways were highly enriched, notably oxidative phosphorylation, thermogenesis, arachidonic acid metabolism, and steroid hormone biosynthesis. Some of these pathways have a role in sperm survival, fertilization, and early embryo development. In conclusion, the findings of our study demonstrate for the first time that bovine FF and FF-derived EVs can induce changes in the gene expression of the bovine oviductal cells which, although observed in vitro, may be reflective of in vivo responses which may contribute to a favorable periconceptional microenvironment for sperm survival, fertilization, and early embryo development. View Full-Text
Keywords: follicular fluid; extracellular vesicles; oviductal epithelial cells; gene expression; fertilization; embryonic development follicular fluid; extracellular vesicles; oviductal epithelial cells; gene expression; fertilization; embryonic development
Show Figures

Figure 1

MDPI and ACS Style

Hasan, M.M.; Viil, J.; Lättekivi, F.; Ord, J.; Reshi, Q.U.A.; Jääger, K.; Velthut-Meikas, A.; Andronowska, A.; Jaakma, Ü.; Salumets, A.; Fazeli, A. Bovine Follicular Fluid and Extracellular Vesicles Derived from Follicular Fluid Alter the Bovine Oviductal Epithelial Cells Transcriptome. Int. J. Mol. Sci. 2020, 21, 5365. https://doi.org/10.3390/ijms21155365

AMA Style

Hasan MM, Viil J, Lättekivi F, Ord J, Reshi QUA, Jääger K, Velthut-Meikas A, Andronowska A, Jaakma Ü, Salumets A, Fazeli A. Bovine Follicular Fluid and Extracellular Vesicles Derived from Follicular Fluid Alter the Bovine Oviductal Epithelial Cells Transcriptome. International Journal of Molecular Sciences. 2020; 21(15):5365. https://doi.org/10.3390/ijms21155365

Chicago/Turabian Style

Hasan, Mohammad M., Janeli Viil, Freddy Lättekivi, James Ord, Qurat U.A. Reshi, Kersti Jääger, Agne Velthut-Meikas, Aneta Andronowska, Ülle Jaakma, Andres Salumets, and Alireza Fazeli. 2020. "Bovine Follicular Fluid and Extracellular Vesicles Derived from Follicular Fluid Alter the Bovine Oviductal Epithelial Cells Transcriptome" International Journal of Molecular Sciences 21, no. 15: 5365. https://doi.org/10.3390/ijms21155365

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop