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Sperm Motility of Mice under Simulated Microgravity and Hypergravity

Cell Biophysics Laboratory, State Scientific Center of the Russian Federation, Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe Shosse, Moscow 123007, Russia
Department of Medical and Biological Physics, I. M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya Street, Moscow 119991, Russia
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(14), 5054;
Received: 9 June 2020 / Revised: 25 June 2020 / Accepted: 14 July 2020 / Published: 17 July 2020
(This article belongs to the Special Issue Microgravity and Space Medicine)
For deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse sperm motility and the mechanisms of this change. For 1, 3 and 6 h, mouse sperm samples isolated from the caudal epididymis were subjected to simulated microgravity using a random position machine and 2g hypergravity using a centrifuge. The experimental samples were compared with static and dynamic controls. The sperm motility and the percentage of motile sperm were determined using microscopy and video analysis, cell respiration was determined by polarography, the protein content was assessed by Western blotting and the mRNA levels were determined using qRT-PCR. The results indicated that hypergravity conditions led to more significant changes than simulated microgravity conditions: after 1 h, the speed of sperm movement decreased, and after 3 h, the number of motile cells began to decrease. Under the microgravity model, the speed of movement did not change, but the motile spermatozoa decreased after 6 h of exposure. These changes are likely associated with a change in the structure of the microtubule cytoskeleton, and changes in the energy supply are an adaptive reaction to changes in sperm motility. View Full-Text
Keywords: sperm motility; cell respiration; cytoskeleton; simulated microgravity; hypergravity sperm motility; cell respiration; cytoskeleton; simulated microgravity; hypergravity
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MDPI and ACS Style

Ogneva, I.V.; Usik, M.A.; Biryukov, N.S.; Zhdankina, Y.S. Sperm Motility of Mice under Simulated Microgravity and Hypergravity. Int. J. Mol. Sci. 2020, 21, 5054.

AMA Style

Ogneva IV, Usik MA, Biryukov NS, Zhdankina YS. Sperm Motility of Mice under Simulated Microgravity and Hypergravity. International Journal of Molecular Sciences. 2020; 21(14):5054.

Chicago/Turabian Style

Ogneva, Irina V., Maria A. Usik, Nikolay S. Biryukov, and Yuliya S. Zhdankina 2020. "Sperm Motility of Mice under Simulated Microgravity and Hypergravity" International Journal of Molecular Sciences 21, no. 14: 5054.

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