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Article

Regulation of ABA-Non-Activated SNF1-Related Protein Kinase 2 Signaling Pathways by Phosphatidic Acid

1
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland
2
Structural Biology Group, Biological and Chemical Research Centre, Department of Chemistry, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, Poland
*
Author to whom correspondence should be addressed.
These authors contribute equally to the work.
Int. J. Mol. Sci. 2020, 21(14), 4984; https://doi.org/10.3390/ijms21144984
Received: 16 June 2020 / Revised: 9 July 2020 / Accepted: 12 July 2020 / Published: 15 July 2020
(This article belongs to the Special Issue Kinase Signal Transduction 2.0)
Phosphatidic acid (PA) is involved in the regulation of plant growth and development, as well as responses to various environmental stimuli. Several PA targets in plant cells were identified, including two SNF1-related protein kinases 2 (SnRK2s), SnRK2.10 and SnRK2.4, which are not activated by abscisic acid (ABA). Here, we investigated the effects of PA on various elements of ABA-non-activated SnRK2 signaling. PA 16:0/18:1 was found to modulate the SnRK2 structure and the phosphorylation of some SnRK2 targets. Conversely, phosphorylation by the ABA-non-activated SnRK2s, of one of such targets, dehydrin Early Responsive to Dehydration 14 (ERD14), affects its interaction with PA and subcellular localization. Moreover, PA 16:0/18:1 modulates the activity and/or localization of negative regulators of the ABA-non-activated SnRK2s, not only of the ABA insensitive 1 (ABI1) phosphatase, which was identified earlier, but also of another protein phosphatase 2C, PP2CA. The activity of both phosphatases was inhibited by about 50% in the presence of 50 μM PA. PA 16:0/18:1 also impacts the phosphorylation and subcellular localization of SnRK2-interacting calcium sensor, known to inhibit SnRK2 activity in a calcium-dependent manner. Thus, PA was found to regulate ABA-non-activated SnRK2 signaling at several levels: the activity, phosphorylation status and/or localization of SnRK2 cellular partners. View Full-Text
Keywords: Arabidopsis thaliana; SnRK2-interacting calcium sensor; SCS; dehydrins; ERD14; PP2CA; SnRK2; SNF1-related protein kinases; phosphatidic acid; PA Arabidopsis thaliana; SnRK2-interacting calcium sensor; SCS; dehydrins; ERD14; PP2CA; SnRK2; SNF1-related protein kinases; phosphatidic acid; PA
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MDPI and ACS Style

Klimecka, M.; Bucholc, M.; Maszkowska, J.; Krzywińska, E.; Goch, G.; Lichocka, M.; Szczegielniak, J.; Dobrowolska, G. Regulation of ABA-Non-Activated SNF1-Related Protein Kinase 2 Signaling Pathways by Phosphatidic Acid. Int. J. Mol. Sci. 2020, 21, 4984. https://doi.org/10.3390/ijms21144984

AMA Style

Klimecka M, Bucholc M, Maszkowska J, Krzywińska E, Goch G, Lichocka M, Szczegielniak J, Dobrowolska G. Regulation of ABA-Non-Activated SNF1-Related Protein Kinase 2 Signaling Pathways by Phosphatidic Acid. International Journal of Molecular Sciences. 2020; 21(14):4984. https://doi.org/10.3390/ijms21144984

Chicago/Turabian Style

Klimecka, Maria, Maria Bucholc, Justyna Maszkowska, Ewa Krzywińska, Grażyna Goch, Małgorzata Lichocka, Jadwiga Szczegielniak, and Grażyna Dobrowolska. 2020. "Regulation of ABA-Non-Activated SNF1-Related Protein Kinase 2 Signaling Pathways by Phosphatidic Acid" International Journal of Molecular Sciences 21, no. 14: 4984. https://doi.org/10.3390/ijms21144984

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