Angiotensin II Infusion Leads to Aortic Dissection in LRP8 Deficient Mice
Abstract
:1. Introduction
2. Results
2.1. AngII Induces Vascular Dysfunction and Immune Cell Infiltration in LRP8+/+ and LRP8−/− Mice
2.2. LRP8 Deficient Mice Infused with AngII Develop Aortic Dissections
2.3. AngII-Induced Aortic Dissections are Driven by LRP8 Deficient Bone Marrow Derived Cells
3. Discussion
4. Materials and Methods
4.1. Animals, In Vivo Treatment and Blood Pressure Recording
4.2. Vascular Reactivity Studies
4.3. Intravital Fluorescence Microscopy
4.4. Flow Cytometric Analysis of Aortic Lysates
4.5. Picro-Sirius Red Staining
4.6. mRNA Expression Analysis
4.7. Bone Marrow Transplantation
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ACh | Acetylcholine |
AngII | Angiotensin II |
ApoER2 | Apolipoprotein E receptor 2 |
FXI | Factor XI |
GPIbα | Glycoprotein Ib alpha |
LRP8 | Low-density lipoprotein receptor-related protein 8 |
References
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LRP8+/+ | LRP8+/+ + AngII | LRP8−/− | LRP8−/− + AngII | |
---|---|---|---|---|
n | 7 | 6 | 6 | 6 |
WBC (103/µL) | 4.0 ± 0.4 | 4.5 ± 0.6 | 4.7 ± 0.6 | 4.6 ± 0.5 |
RBC (106/µL) | 8.0 ± 0.1 | 8.8 ± 0.3 * | 8.3 ± 0.1 | 9.1 ± 0.4 |
HGB (g/dL) | 12.0 ± 0.1 | 12.8 ± 0.4 | 12.0 ± 0.2 | 13.1 ± 0.5 |
HCT (%) | 42 ± 0.4 | 46 ± 1.4 * | 43 ± 0.6 | 47 ± 2.1 |
Platelets (103/µL) | 967 ± 23 | 1035 ± 90 | 1001 ± 79 | 929 ± 74 |
MPV (fl) | 5.7 ± 0.1 | 6.0 ± 0.1 * | 5.7 ± 0.1 | 5.9 ± 0.1 * |
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Lagrange, J.; Finger, S.; Kossmann, S.; Garlapati, V.; Ruf, W.; Wenzel, P. Angiotensin II Infusion Leads to Aortic Dissection in LRP8 Deficient Mice. Int. J. Mol. Sci. 2020, 21, 4916. https://doi.org/10.3390/ijms21144916
Lagrange J, Finger S, Kossmann S, Garlapati V, Ruf W, Wenzel P. Angiotensin II Infusion Leads to Aortic Dissection in LRP8 Deficient Mice. International Journal of Molecular Sciences. 2020; 21(14):4916. https://doi.org/10.3390/ijms21144916
Chicago/Turabian StyleLagrange, Jeremy, Stefanie Finger, Sabine Kossmann, Venkata Garlapati, Wolfram Ruf, and Philip Wenzel. 2020. "Angiotensin II Infusion Leads to Aortic Dissection in LRP8 Deficient Mice" International Journal of Molecular Sciences 21, no. 14: 4916. https://doi.org/10.3390/ijms21144916