Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H+-ATPase Rich Ionocytes

Arginine vasopressin (Avp) is a conserved pleiotropic hormone that is known to regulate both water reabsorption and ion balance; however, many of the mechanisms underlying its effects remain unclear. Here, we used zebrafish embryos to investigate how Avp modulates ion and acid–base homeostasis. After incubating embryos in double-deionized water for 24 h, avp mRNA expression levels were significantly upregulated. Knockdown of Avp protein expression by an antisense morpholino oligonucleotide (MO) reduced the expression of ionocyte-related genes and downregulated whole-body Cl⁻ content and H⁺ secretion, while Na⁺ and Ca²⁺ levels were not affected. Incubation of Avp antagonist SR49059 also downregulated the mRNA expression of sodium chloride cotransporter 2b (ncc2b), which is a transporter responsible for Cl⁻ uptake. Correspondingly, avp morphants showed lower NCC and H⁺-ATPase rich (HR) cell numbers, but Na⁺/K⁺-ATPase rich (NaR) cell numbers remained unchanged. avp MO also downregulated the numbers of foxi3a- and p63-expressing cells. Finally, the mRNA expression levels of calcitonin gene-related peptide (cgrp) and its receptor, calcitonin receptor-like 1 (crlr1), were downregulated in avp morphants, suggesting that Avp might affect Cgrp and Crlr1 for modulating Cl⁻ balance. Together, our results reveal a molecular/cellular pathway through which Avp regulates ion and acid–base balance, providing new insights into its function. View Full-Text

Keywords: vasopressin; ionocyte; and ion regulation

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Tong, S.-K.; Lee, H.-L.; Lee, Y.-C.; Wu, L.-C.; Tsou, Y.-L.; Lu, S.-W.; Shih, S.-W.; Hwang, P.-P.; Chou, M.-Y. Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H⁺-ATPase Rich Ionocytes. Int. J. Mol. Sci. 2020, 21, 3957. https://doi.org/10.3390/ijms21113957

AMA Style

Tong S-K, Lee H-L, Lee Y-C, Wu L-C, Tsou Y-L, Lu S-W, Shih S-W, Hwang P-P, Chou M-Y. Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H⁺-ATPase Rich Ionocytes. International Journal of Molecular Sciences. 2020; 21(11):3957. https://doi.org/10.3390/ijms21113957

Chicago/Turabian Style

Tong, Sok-Keng, Hung-Ling Lee, Yi-Chun Lee, Liang-Chun Wu, Yi-Ling Tsou, Shao-Wei Lu, Shang-Wu Shih, Pung-Pung Hwang, and Ming-Yi Chou. 2020. "Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H⁺-ATPase Rich Ionocytes" International Journal of Molecular Sciences 21, no. 11: 3957. https://doi.org/10.3390/ijms21113957

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Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H⁺-ATPase Rich Ionocytes

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