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Article

Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H+-ATPase Rich Ionocytes

1
Department of Life Science, National Taiwan University, Taipei 10617, Taiwan
2
Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 11529, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(11), 3957; https://doi.org/10.3390/ijms21113957
Received: 17 May 2020 / Revised: 26 May 2020 / Accepted: 30 May 2020 / Published: 31 May 2020
(This article belongs to the Special Issue Molecular Biology of the Pituitary)
Arginine vasopressin (Avp) is a conserved pleiotropic hormone that is known to regulate both water reabsorption and ion balance; however, many of the mechanisms underlying its effects remain unclear. Here, we used zebrafish embryos to investigate how Avp modulates ion and acid–base homeostasis. After incubating embryos in double-deionized water for 24 h, avp mRNA expression levels were significantly upregulated. Knockdown of Avp protein expression by an antisense morpholino oligonucleotide (MO) reduced the expression of ionocyte-related genes and downregulated whole-body Cl content and H+ secretion, while Na+ and Ca2+ levels were not affected. Incubation of Avp antagonist SR49059 also downregulated the mRNA expression of sodium chloride cotransporter 2b (ncc2b), which is a transporter responsible for Cl uptake. Correspondingly, avp morphants showed lower NCC and H+-ATPase rich (HR) cell numbers, but Na+/K+-ATPase rich (NaR) cell numbers remained unchanged. avp MO also downregulated the numbers of foxi3a- and p63-expressing cells. Finally, the mRNA expression levels of calcitonin gene-related peptide (cgrp) and its receptor, calcitonin receptor-like 1 (crlr1), were downregulated in avp morphants, suggesting that Avp might affect Cgrp and Crlr1 for modulating Cl balance. Together, our results reveal a molecular/cellular pathway through which Avp regulates ion and acid–base balance, providing new insights into its function. View Full-Text
Keywords: vasopressin; ionocyte; and ion regulation vasopressin; ionocyte; and ion regulation
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MDPI and ACS Style

Tong, S.-K.; Lee, H.-L.; Lee, Y.-C.; Wu, L.-C.; Tsou, Y.-L.; Lu, S.-W.; Shih, S.-W.; Hwang, P.-P.; Chou, M.-Y. Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H+-ATPase Rich Ionocytes. Int. J. Mol. Sci. 2020, 21, 3957. https://doi.org/10.3390/ijms21113957

AMA Style

Tong S-K, Lee H-L, Lee Y-C, Wu L-C, Tsou Y-L, Lu S-W, Shih S-W, Hwang P-P, Chou M-Y. Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H+-ATPase Rich Ionocytes. International Journal of Molecular Sciences. 2020; 21(11):3957. https://doi.org/10.3390/ijms21113957

Chicago/Turabian Style

Tong, Sok-Keng, Hung-Ling Lee, Yi-Chun Lee, Liang-Chun Wu, Yi-Ling Tsou, Shao-Wei Lu, Shang-Wu Shih, Pung-Pung Hwang, and Ming-Yi Chou. 2020. "Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H+-ATPase Rich Ionocytes" International Journal of Molecular Sciences 21, no. 11: 3957. https://doi.org/10.3390/ijms21113957

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