Next Article in Journal
Proprotein Convertase Subtilisin/Kexin Type 9, Angiopoietin-Like Protein 8, Sortilin, and Cholesteryl Ester Transfer Protein—Friends of Foes for Psoriatic Patients at the Risk of Developing Cardiometabolic Syndrome?
Previous Article in Journal
Regulation of a PRMT5/NF-κB Axis by Phosphorylation of PRMT5 at Serine 15 in Colorectal Cancer
Open AccessArticle

Neutrophils Modulate Fibroblast Function and Promote Healing and Scar Formation after Murine Myocardial Infarction

1
Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, 52074 Aachen, Germany
2
Victor Babes National Institute of Pathology, 050096 Bucharest, Romania
3
Department of Anesthesiology, University Hospital, RWTH Aachen University, 52074 Aachen, Germany
4
Institut für Pharmakologie und Klinische Pharmakologie, University Hospital, 40225 Düsseldorf, Germany
5
Experimental Vascular Pathology, Cardiovascular Research Institute Maastricht (CARIM), University of Maastricht, 6200 Maastricht, The Netherlands
6
Human Genetic Laboratory, University of Medicine and Pharmacy, 200642 Craiova, Romania
7
Cardiovascular & Metabolic Disorders Program, Duke-National University of Singapore Medical School, Singapore 169857, Singapore
8
National Heart Research Institute Singapore, National Heart Centre, Singapore 169609, Singapore
9
Yong Loo Lin School of Medicine, National University Singapore, Singapore 169857, Singapore
10
The Hatter Cardiovascular Institute, University College London, London WC1E 6BT, UK
11
Cardiovascular Research Center, College of Medical and Health Sciences, Asia University, Taichung 41354, Taiwan
12
Department of Cardiology, Pulmonology, Angiology and Intensive Care, University Hospital, RWTH Aachen University, 52074 Aachen, Germany
*
Author to whom correspondence should be addressed.
The authors contributed equally to this manuscript.
Int. J. Mol. Sci. 2020, 21(10), 3685; https://doi.org/10.3390/ijms21103685
Received: 16 April 2020 / Revised: 19 May 2020 / Accepted: 19 May 2020 / Published: 23 May 2020
(This article belongs to the Special Issue Neutrophils in Cardiovascular Diseases)
Aim: Recruitment of neutrophils to the heart following acute myocardial infarction (MI) initiates inflammation and contributes to adverse post-infarct left ventricular (LV) remodeling. However, therapeutic inhibition of neutrophil recruitment into the infarct zone has not been beneficial in MI patients, suggesting a possible dual role for neutrophils in inflammation and repair following MI. Here, we investigate the effect of neutrophils on cardiac fibroblast function following MI. Methods and Results: We found that co-incubating neutrophils with isolated cardiac fibroblasts enhanced the production of provisional extracellular matrix proteins and reduced collagen synthesis when compared to control or co-incubation with mononuclear cells. Furthermore, we showed that neutrophils are required to induce the transient up-regulation of transforming growth factor (TGF)-ß1 expression in fibroblasts, a key requirement for terminating the pro-inflammatory phase and allowing the reparatory phase to form a mature scar after MI. Conclusion: Neutrophils are essential for both initiation and termination of inflammatory events that control and modulate the healing process after MI. Therefore, one should exercise caution when testing therapeutic strategies to inhibit neutrophil recruitment into the infarct zone in MI patients. View Full-Text
Keywords: myocardial infarction; inflammation; neutrophils; fibroblasts; extracellular matrix formation; scar formation myocardial infarction; inflammation; neutrophils; fibroblasts; extracellular matrix formation; scar formation
Show Figures

Figure 1

MDPI and ACS Style

Curaj, A.; Schumacher, D.; Rusu, M.; Staudt, M.; Li, X.; Simsekyilmaz, S.; Jankowski, V.; Jankowski, J.; Dumitraşcu, A.R.; Hausenloy, D.J.; Schuh, A.; Liehn, E.A. Neutrophils Modulate Fibroblast Function and Promote Healing and Scar Formation after Murine Myocardial Infarction . Int. J. Mol. Sci. 2020, 21, 3685.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop