Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive degeneration of motoneurons (MN) in the primary motor cortex, brainstem, and spinal cord [1
]. The disease is sporadic in 90%–95% of cases, while it is familial in the remaining cases (with an autosomal dominant, autosomal recessive, or X-linked inheritance), and 20% of the familial ALS are due to mutations in the gene encoding for Cu2+
superoxide dismutase (SOD1) [2
]. The mechanisms underlying the neurodegeneration of the disease are complex and still need to be clarified [3
Transgenic mice overexpressing the human SOD1 gene with a reported G93A mutation (SOD1(G93A)) is the most widely used murine model to study ALS. This model exhibits the clinical signs and the pathological features of human patients, and provides an optimal in vivo model for investigating the pathogenesis of disease and for testing new therapeutic approaches [4
Due to the complexity of the pathogenetic mechanisms involved in this neurodegenerative disorder, to date, there is no effective treatment available [5
]. A promising therapeutic approach in neurodegenerative diseases is represented by stem cells that are able to self-renew, differentiate, and home to damage sites, which contribute to tissue repair and regeneration. Mesenchymal stem cells (MSC) are considered to be the best candidates and, among these, adipose-derived stem cells (ASCs) are accessible in large amounts and allow an easily autologous cell transplantation [6
Several studies, including ours, have already demonstrated the beneficial effects of stem cells after their transplantation in murine models of ALS. The cells delayed the symptom progression of the disease, improved the function of the neuromuscular junction, and decreased the inflammatory response of the animals, indicating that they could promote neuroprotection [7
]. However, despite the encouraging results concerning stem cell treatment, migration, engraftment, and differentiation of stem cells at the sites of injury were reported rarely. For these reasons, the hypothesis that stem cells exert their therapeutic activity through secreted molecules and extracellular vesicles is plausible [3
]. Among extracellular vesicles, exosomes are 50–150 nm in diameter and play important roles in intercellular communication, recapitulating the effect of stem cell transplantation by transferring biologically active molecules to recipient cells, altering their gene expression and behavior [12
]. Several studies have suggested that exosomes isolated from stem cells are involved in neuronal protection, nerve regeneration, neurological recovery, and synaptic plasticity. These vesicles can be exploited as a therapy instead of their derived parental cells, avoiding the limitations and risks associated with cell transplantation (reviewed in [13
In the present study, we tested the neuroprotective effect of exosomes isolated from ASCs (ASC-exosomes) in the SOD1(G93A) murine model of ALS. Moreover, we compared the effect of two different routes of exosomes administration, i.e., intravenous (i.v.) and intranasal (i.n.). First, we administered ASC-exosomes i.v., as previously done with ASCs [8
]. Furthermore, we assessed the i.n. delivery, since it is easily transferable to ALS patients avoiding their hospitalization and represents a noninvasive way to deliver exosomes directly to the CNS.
In this study, we demonstrated, for the first time, that repeated administration of exosomes (i.v. or i.n.) improved the motor performance; protected the lumbar MN, neuromuscular junction (NMJ), and muscle; and decreased the glial cells activation in treated SOD1(G93A) mice. Moreover, we tested the capacity of exosomes to reach the CNS and the lesion sites. For this purpose, we used magnetic resonance imaging (MRI) and exosomes labeled with ultra-small superparamagnetic iron oxide nanoparticles (USPIO) as contrast agents to produce a detectable change in signal intensity [14
]. After i.n. administration, we detected labeled exosomes (exosomes-USPIO) in the brain, indicating that the vesicles reached the CNS and accumulated in the typical lesioned brainstem motor nuclei of the SOD1(G93A) mice.
Taken together, these results prove that ASC-exosomes are able to slow down the clinical progression of ALS; exert a protective effect on MN, NMJ, and muscle; and reduce glial cells activation. Moreover, ASC-exosomes home to lesioned regions of animal brain. These data contribute by providing additional knowledge for the promising use of ASC-exosomes as a novel cell-free therapy in ALS.
The development of new therapies for ALS is crucial to improve the quality of life of patients and extend their survival. Many studies have been conducted in order to develop pharmacological/cell therapies [17
], but the challenge is still open. It is known that ALS is a multifactorial disease, since different pathogenetic mechanisms are involved in motoneuron degeneration [3
]. All pharmacological treatments tested for ALS have usually been focused on single or few altered cellular pathways, and all clinical trials have failed to show any significant results [5
]. The stem cell therapy has the advantage that it can act via multiple mechanisms, simultaneously counteracting different pathogenetic pathways and providing a more effective treatment for ALS patients [3
]. The therapeutic efficacy of stem cells of different origins has been evaluated in experimental models of ALS, with encouraging results [7
It has emerged that the beneficial function of MSC could be mediated by the release of extracellular vesicles, as exosomes, rather than their engraftment and differentiation [20
]. A therapy based on the use of exosomes would prevent all the risk associated with cells transplantation and would require administering only the fraction responsible for the beneficial effect of stem cells [21
]. The exosomes transfer functional molecules (in particular proteins, mRNA, and miRNA) to other cells, modulating their activity, and maintaining the integrity of their cargos from proteases and nucleases degradation. Moreover, among the extracellular vesicles, exosomes are the only ones that can cross the blood-brain barrier thanks to their small dimension, a feature that renders these vesicles more interesting as a therapeutic approach in several neurodegenerative diseases [13
]. With evidence that extracellular vesicles recapitulate the effect of stem cells, several works have tested their therapeutic efficacy in neurodegenerative disease models. The exosomes reduce the pathological accumulation of the β-amyloid peptide in an in vitro model of Alzheimer’s disease stimulating their proteolysis [22
], protecting dopaminergic neurons from apoptosis after oxidative stress [23
], and showing a neuroprotective effect in an in vitro model of ALS [24
]. In this regard, the proteomic analysis of exosomes, correlating the protein content to the anti-apoptotic effect, was useful to understand the mechanisms by which ASC-exosomes exert their beneficial effect [25
]. In the in vivo model of the disease, exosomes are able to promote neural plasticity and functional recovery after stroke in rats via the transfer of miRNA-133b [26
], induce neurite outgrowth and improve neuroregeneration after traumatic brain injury in C57BL6 mice [27
], and ameliorate chronic experimental autoimmune encephalomyelitis [28
]. Moreover, exosomes isolated from a different source of MSC alleviate inflammation and prevent cognitive and memory impairments after status epilepticus [29
], alleviate neuroinflammation and reduce β-amyloid accumulation in a mouse model of Alzheimer’s disease [30
], and suppress inflammatory response enhancing the regeneration of mice spinal cord after injury [31
In the present study, we investigated whether ASC-exosomes exert a protective effect in vivo in transgenic SOD1(G93A) mice of ALS. We decided to administer ASC-exosomes every four days, since this experimental paradigm presents an optimal compromise between frequency and route of administration, above all for chronic intravenous administration in which more frequent injections could be harmful to the mice and not allow the regeneration of epithelial vein tissue. We demonstrated that repeated i.v. or i.n. administrations of ASC-exosomes improved the motor performance from week 11 to week 15 of life as compared with the controls (PBS-treated mice). From week 17 of life, the beneficial effect observed in ASC-exosomes treated mice disappeared and no differences in motor performance were observed as compared with the PBS group. This data indicates that, at the late phase of the disease, when the interaction between MN and NMJ is highly compromised [32
], the dose of ASC-exosomes used was inefficient, underling that this treatment could be dose-dependent, as already demonstrated in other experimental studies [33
As reported in the literature, in SOD1(G93A) mice, there is a progressive lumbar MN degeneration during the progression of the pathology [36
]. This result is also confirmed by our study, which showe 22.9% MN loss at week 15 and 56.2% MN loss at week 19. Interestingly, we demonstrated that ASC-exosomes treatment was able to protect lumbar MN degeneration during the disease progression, showing a progressively increased percentage of MN survival between 15 and 19 weeks of life, being significant at the last time point. In addition to survival, our data suggest that ASC-exosomes improves the function of MN, as indicated by a significantly higher number of preserved NMJ and skeletal muscle fibers at 15 weeks of life.
Neuroinflammation, determined by astrogliosis, microgliosis, and infiltration of immune cells, is a common characteristic of ALS and other neurodegenerative diseases. In particular, through the release of soluble factors, astrocytes exert a potent toxic property on MN, contributing to their neurodegeneration [37
]. It has been reported that exosomes derived from stem cells suppress the activation of neurotoxic astrocytes after traumatic spinal cord injury, ischemia, and epilepsy [31
]. In our study, we demonstrate that ASC-exosomes are able to decrease astrocytes activation but not the total number of activated microglial cells.
The route of delivery of ASC-exosomes could also have profound relevance. In this study, we tested the intranasal administration, which represented a noninvasive procedure that allowed repetitive dispensation and an efficient delivery of the vesicles to the brain. These characteristics make the i.n. administration an efficient approach to deliver exosomes to the regions involved in this neurological disease, since earlier studies have shown that the systemic administration of exosomes determined an accumulation in the spleen and the liver [41
]. Moreover, these features would allow an easy and more effective transferability of ASC-exosomes treatment for patients with neurological diseases.
To understand the migration and homing abilities of exosomes in the brain after i.n. administration, a recent study reported that bone marrow MSC-exosomes selectively targeted lesioned areas in murine models of stroke, Parkinson’s disease, Alzheimer’s disease, and autism [43
]. In this study, we investigated the distribution of ASC-exosomes by MRI after 3 and 24 h of i.n. administration. We demonstrated that ASC-exosomes reached the injured brain area of SOD1(G93A) mice, the brainstem regions corresponding to the trigeminal and the facial motor nuclei [15
]. This indicates that injured signals could play a role in attracting ASC-exosomes in the specific injured brain for a limited period of time. Indeed, hypointense signal indicating the presence of labeled exosomes was not detected after 24 h. This could indicate that ASC-exosomes spread through the CNS and reach the lesioned spinal cord. Data concerning the homing of ASC-exosomes in the spinal cord could elucidate if their action is direct on MN and astrocyte in this site of CNS. Moreover, it remains to be clarified which cells uptake ASC-exosomes. The elucidation of these mechanisms would contribute to open the way to the application of exosomes therapy in ALS and other neurodegenerative conditions.
4. Materials and Methods
4.1. ASC Cell Cultures
Murine ASCs were obtained from inguinal adipose tissues of C57Bl6/J mice (Charles River Laboratories, Sant’Angelo Lodigiano, Italy). The isolation of stromal vascular fraction was carried out as previously described [44
]. The extracellular matrix was incubated in Hank’s Balanced Salt Solution (Life Technologies Italia, Milan, Italy) with collagenase type I (Life Technologies Italia, Milan, Italy) and bovine serum albumin (BSA, AppliChem Nova Chimica Srl, Milan, Italy), centrifuged, and suspended in NH4
Cl. The fraction was centrifuged again and filtered to remove cell debris. Then, cells were cultured using DMEM, 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (all from GIBCO Life Technologies, Milan, Italy) and incubated at 37 °C/5% CO2
. Murine ASCs were recognized by immunophenotype using monoclonal antibodies specific for CD106, CD9, CD44, CD80, and CD138, and by the absence of hematopoietic and endothelial markers (as CD45, CD11c, and CD31), as previously described [8
Exosomes were isolated from the culture medium of 107ASC. The cells were cultured to confluence and 48 h of FBS deprivation was made to avoid any contamination of vesicles from serum.
To isolate labeled exosomes-USPIO, 107
ASC were cultured to confluence, and then incubated with 200 µg Fe/mL of USPIO for 24 h, followed by FBS deprivation for 48 h, as previously described [14
Then, Ccll culture supernatant was collected and exosomes (labeled or unlabeled) were obtained using Pure Exo Exosomes Isolation Kit (101Bio, Montain View, CA, USA), following the manufacturer’s protocol. The protein content of exosomes was determined by Bicinchoninic Protein Assay using the manufacturer’s protocol (Thermo Scientific™, Milan, Italy BCA™ Protein Assay). The size and concentration of ASC-exosomes were assessed by QNano instrument.
Concerning exosomes-USPIO, the quantification of iron nanoparticles was performed as previously described in [45
The ASC-exosomes were used to evaluate their neuroprotection in vivo, the exosomes-USPIO were used in MRI to detect their presence in the CNS after in vivo administration.
4.3. Electron Microscopy and Western Blot
Electron microscopy of exosomes was performed as previously described [24
]. Exosome pellets were fixed in 2% glutaraldehyde in Sorensen buffer (pH 7.4) for 2 h, and then postfixed in 1% osmium tetroxide (OsO4
) in aqueous solution for 2 h. The sample was dehydrated in graded concentrations of acetone and embedded in Epon-Araldite mixture (Electron Microscopy Sciences, Fort Washington, PA, USA). The semithin sections (1 µm in thickness) were examined by light microscopy (Olympus BX51, Olympus Optical, Hamburg, Germany) and stained with toluidine blue. The ultrathin sections were cut at a 70 nm thickness, placed on Cu/Rh grids with Ultracut E (Reichert, Wien, Austria), and observed with transmission electron microscopy (TEM) using a Morgagni 268D electron microscope (Philips).
Western blot hybridization was performed as previously described [24
]. Proteins were denatured, separated on 4%–12% polyacrylamide gels and transferred onto a nitrocellulose membrane. Antibodies against murine HSP70 (70 kDa, 1.100 HSP70 (K-20): sc-1060 Santa Cruz Biotechnology, DBA Italia Srl, Milan, Italy) and CD9 (25 kDa, 1:100 MM2/57, Millipore CBL-162) were used, followed by HRP-conjugated secondary antibodies (Dako Agilent, Milaln, Italy). ASC lysates were used as a positive control. Then, the blot was incubated with a chemiluminescent HRP substrate and detected with G:BOX F3 GeneSys (Syngene, Cambridge, UK).
Experiments were performed using transgenic mice overexpressing human SOD1 carrying a Gly93-Ala mutation (SOD1(G93A)) (strain designation B6SJL–TgN[SOD1–G93A]1Gur, stock number 002726) and wild-type (WT) mice (B6SJL) obtained from Jackson Laboratories (Bar Harbor, ME, USA). Animals were maintained under controlled environmental conditions (temperature, humidity, 12 h/12 h light/dark cycle, water, and food ad libitum) and veterinarian assistance. The research complies with the commonly accepted “3Rs”. The experiments were performed with the approval of the Animal Care and Use Committee of the University of Verona (CIRSAL), and the Italian Ministry of Health, in strict adherence to the European Communities Council (2010/63/EEC) directives (project identification code 710/2018-PR, approved 24/09/2018), minimizing the number of animals used and avoiding their suffering. Transgenic mice were identified by a polymerase chain reaction specific for human SOD1 gene (primers for SOD1 gene were forward (113) 5′-CATCAGCCCTAATCCATCTGA-3′ and reverse (114) 5′-CGCGACTAACAATCAAAGTGA-3′; while for the housekeeping gene interleukin-2 receptor (IL-2R) the primers were forward (42) 5′-CTAGGCCACAGAATTGAAAGATCT-3′; reverse (43) 5′-GTAGGTGGAAATTCTAGCATCATCC-3′).
4.5. Motor Tests
The progression of the disease was monitored starting at 50 days by careful motor performance examination. In order to test the efficacy of the treatment, SOD1(G93A) mice were weekly evaluated blinded for body weight, neurological score test, paw grip endurance (PaGE) test, and rotarod test. The neurological score test was evaluated as follows: 4, normal (no sign of motor dysfunction); 3, hind limb tremors were present when the mice were suspended by tail; 2, gait abnormalities; 1, dragging at least one hind limb; and 0, inability to right itself in 30 sec when the animal was placed in the supine position. The PaGE test was used to assess the grip strength of the animals. The test was performed by placing the animal on a metal grid and quickly turning it over. The score was measured by the length of time that the mouse was able to grip onto the grid. Each mouse was given up to two attempts to hold onto the inverted grid with an arbitrary cut-off time of 120 s. The rotarod test was used to assess the motor coordination of the animals. The mice were placed in a rotor tube (Acceler Rota-Rod 7650, Ugo Basile, Varese, Italy) at a constant speed of 16 rpm. The cut-off time was settled at 180 sec and three attempts were given to mice that failed the test, with a resting phase of 5 min. The longest latency time was registered.
The animals failed the PaGE test or the rotarod test when they were not able to reach the cut-off time. The onset was established when the mouse failed the PaGE or rotarod test. When the neurological score was equal to zero, the animals were sacrificed, and the survival time was recorded.
4.6. Animal Treatments
To test the therapeutic efficacy of ASC-exosomes, a total of 56 SOD1(G93A) male mice were used in the experimental paradigm. The ASC-exosomes were administered in SOD1(G93A) mice intravenously (10 treated mice and 10 control mice) or intranasally (10 treated mice and 10 control mice). The ASC-exosomes administrations were performed from the onset of the clinical sign until the end stage, every 4 days. For each injection, the treated mice were injected with 1 µg of ASC-exosomes, while the control mice received sterile PBS. The total amount of injected solution was 100 µL for an intravenous injection and 10 µL for intranasal administration.
A third group of animals was used. These SOD1(G93A) mice (8 treated mice and 8 control mice) received ASC-exosomes intranasally every 4 days but, in this case, the administrations were performed from the onset of the clinical sign until 15 weeks of life. This time point represents an intermediate point between the onset and the end stage of the disease, when the motor performance was statistically significant different between the control versus the treated groups.
4.7. Lumbar Spinal Cord Motoneurons Stereological Count
At the end stage (19 weeks) or at 15 weeks of life, the SOD1(G93A) mice (n = 5 per group) were deeply anesthetized and transcardially perfused with PBS 0.1 M followed by paraformaldehyde 4%. The spinal cord was dissected out and 2 h of post-fixation was performed. The lumbar tract was soaked in 30% sucrose, included in OCT and serially cut at 15 µm with cryostat apparatus. The sections were mounted on Surgipath® Apex™ Superior Adhesive Slides (3800080E, Leica Biosystems Italia, Milan, Italy).
For Nissl staining, the slides were air-dried, and then hydrated with H2O for 30 sec. The sections were stained with 0.2% cresyl violet solution for 8 min and gradually placed into increasing concentrations of ethanol, cleared with xylene, mounted with Entelan and covered with a cover glass. The MN of the ventral horn in the lumbar spinal cord tract (lateral and medial MN of L1-L5 segments) were counted blinded every 100 µm (every 6/7 slide) by the operator using a computer-assisted microscope (Olympus BX6 with Retiga 2000R camera, Center Valley, PA) with the Stereoinvestigator software (MicroBrightField, Williston, VT, USA) at 40× magnification. Cells with nucleoli on the plane of focus, size and shape typical of MN were counted. The values from the sections were computed for the summation, the mean number was then computed from the average number derived from each animal.
4.8. Immunohistochemistry of Lumbar Spinal Cord
To investigate the activation of astrocytes and microglia cells in the lumbar tract of the spinal cord, immunohistochemistry for light microscopy was performed. The sections were incubated for 10 min in 3% H2O2 to quench endogenous peroxidase and preincubated for 1 h in 5% of NGS in PBS and 1% BSA. The slides were incubated overnight in anti-mouse GFAP or Iba1 antibodies to recognize astrocytes and microglia, respectively, (GFAP 1:500, Z0334 Dako; Iba1 1:500 019–19741 Wako) in 1% NGS in PBS. The sections were washed and incubated for 1 h in biotinylated goat anti-rabbit IgG (1:100, Vector Laboratories). The avidin-biotin peroxidase kit (ABC kit; Vector) and Novared kit (Vector) as signal revelation system was used. After mounting on slides, the sections were dehydrated through increasing grades of ethanol, cleared in xylene, and coverslipped with Entellan (Merck, Darmstadt, Germany). For the analysis, cells were counted every 100 µm for a total of 30 sections for each animal. The astrocytes and microglial cells (GFAP or CD11b labeled cells) of the lumbar tract (L1-L5) were visualized and counted using a computer-assisted microscope (Olympus BX6 with Retiga 2000R camera) with the Stereoinvestigator software (MicroBrightField, Williston, VT, USA).
4.9. Immunohistochemistry of Neuromuscular Junction and Hematoxylin-eosin Staining
WT (n = 5) and SOD1(G93A) mice PBS- or exosomes-treated (n = 5 per group) were deeply anesthetized and transcardially perfused with PBS 0.1 M followed by paraformaldehyde 4%. The hindlimb gastrocnemius muscle was dissected out, post-fixed for 2 h, soaked in 30% sucrose, included in OCT, and longitudinally cut at 20 µm with cryostat apparatus. The sections were mounted on Surgipath® Apex™ Superior Adhesive Slides (3800080E, Leica Biosystems Italia, Milan, Italy). The sections were washed with PBS Triton for 20 min and stained for postsynaptic acetylcholine receptors using CF543 conjugates α-bungarotoxin (αBTx, 1:500, Biotium, DBA Italia Srl, Milan, Italy) for 25 min at room temperature. The sections were washed and incubated for 1 h in 10% NDS and 0.3% Triton X-100 in PBS. Presynaptic motor terminals were labeled using anti-neurofilament H (NF-H; 1:100, Chemicon, Merck, Milan, Italia) overnight at 4 °C. After washing, the sections were incubated with species-specific Alexa Fluor 488 secondary antibody in 2% NDS in PBS (1:1000; Invitrogen, Thermo Fisher Scientific, Milan, Italy) for 1 h at room temperature. After washing, the sections were incubated with DAPI (1:1000; Santa Cruz Biotechnology, DBA Italia Srl, Milan, Italy) for 3 min at room temperature. Sections were washed and mounted with Dako Fluorescence Mounted Medium (Agilent, CA; USA) and dried prior to analysis.
For hematoxylin-eosin (HE) staining, the hindlimb gastrocnemius muscle was transversely cut at 20 µm with cryostat apparatus. The sections were stained with hematoxylin for 40 s, washed with tap water, immersed in eosin for 30 s, and washed with tap water. The sections were dehydrated in ascending alcohol solutions, cleared with xylene, and coverslipped with Entellan (Merck, Darmstadt, Germany). The sections were visualized by optical microscopy (Olympus BX63; Olympus Life Science Solutions, Center Valley, PA). A total of 100 fibers were examined for each animal. The image J software was used to measure the area of gastrocnemius muscle fiber in each group.
4.10. Statistical Analysis
Concerning the motor performance, two-way univariate analysis of variance (ANOVA) and Bonferroni post-hoc tests were performed to evaluate differences between the two animal groups. Data are expressed as mean ± standard error of the mean (SEM). Survival data of the animals were analyzed by Gehan–Breslow–Wilcoxon test. The stereological MN count, immunohistochemistry analysis of glial cells, and data of NMJ immunohistochemistry were analyzed using a two-tailed Student’s test to evaluate differences between groups. Data are reported as mean ± SEM. For all statistical analysis and graphs, GraphPad Prism 5 Software was used, and significance was accepted at p < 0.05.
4.11. Exosomes-USPIO or USPIO Administration
To investigate the ASC-exosomes migration in the CNS, the WT mice (n
= 8) and SOD1(G93A) mice (n
= 8) were divided into the following two groups: one group that received exosomes-USPIO and one group that received only USPIO nanoparticles. The administration was performed at 13 weeks of life in SOD1(G93A) mice, and at the same time of life in the WT mice. This time represents the time point when the typical ALS brain lesions are reported by MRI [15
The exosomes-USPIO were administered intranasally at a concentration of 30 µg of exosomal proteins (containing 0.2 µg of Fe), resuspended in 20 µL of PBS. In the animals that received only USPIO nanoparticles, an amount of USPIO containing the same quantity of Fe present in exosomes-USPIO was administered.
4.12. In Vivo MRI
MR images were acquired using a Bruker Tomograph (Bruker, Karlsruhe, Germany) equipped with a 4.7 T, 33 cm bore horizontal magnet (Oxford Ltd., Oxford, UK). The SOD1(G93A) and WT mice were anesthetized using 1% isofluorane inhalation in a mixture of oxygen and nitrogen and were placed in a prone position over a heated bed. Brain images were acquired with a cross-coil configuration, i.e., a volume birdcage coil was used as transmitter, and a surface helmet coil specific for the mouse brain was used as a receiver. The MRI acquisitions were performed before i.n. administration in all animals. At 13 weeks of life, the SOD1(G93A) mice received i.n. USPIO or exosomes-USPIO (6 animals for each group). The WT mice received i.n. USPIO and exosomes-USPIO (6 animals for each group) at the same weeks of life as the SOD1(G93A) mice. The MRI acquisitions were performed after 3 and 24 h of i.n. administration.
In order to detect USPIO and exosomes-USPIO, T2* map images were acquired using a Multi Gradient Echo (MGE) sequence with repetition time (TR) = 2000 ms, echo time (TE) = 3.6 ms, field of view (FOV) = 2 × 1.5 cm2, matrix size (MTX) = 160/120, slice thickness = 0.5 mm; n° of slice = 20°; number of averages = 9; total acquisition time = 27 min.
Moreover, only in the SOD1(G93A) mice, to identify the areas of lesion, high-resolution T2 weighted structural images were acquired using a Rapid Acquisition with Refocused Echoes (RARE) sequence with the following parameters: TR = 5000 ms; TE = 76 ms; FOV = 2.5 × 2.0 cm2; MTX = 160 × 128; slice thickness = 0.5 mm; n° of slice = 20°; RARE factor = 16; number of averages = 24.
4.13. Histological Analysis after Exosomes-USPIO Administration
After in vivo administration of exosomes-USPIO and MRI acquisitions, the presence of USPIO nanoparticles in the brain of the animals, indicated the presence of exosomes, which was confirmed by histological analysis. Mice were sacrificed and the brain was dissected out, fixed in paraformaldehyde 4%, and soaked overnight for cryoprotection in 20% sucrose at 4 °C. All the samples were cut on a cryostat, and transverse sections (25 µm thick) were collected from all the brains. In order to evaluate the presence of USPIO nanoparticles, Prussian blue staining was performed. The sections were incubated with Prussian blue solution (5% hydrochloric acid and 5% potassium ferrocyanide) for 40 min and counterstained with nuclear fast red, for 10 min. The sections were dehydrated through increasing grades of ethanol, cleared in xylene, coverslipped with Entellan (Merck, Darmstadt, Germany), and examined under a light microscope (Olympus BXS1, Center Valley, PA) equipped with a CCD camera. The sections were examined with the brain mouse atlas in order to confirm that the area detected by histological analysis overlapped with the one detected by MRI.