Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green Alga Chlamydomonas reinhardtii
Abstract
:1. Introduction
2. Results
2.1. Chlamydomonas MAPK Family
2.2. Chlamydomonas MAPK Gene Expression
2.3. Study of a Chlamydomonas Mapk8 Mutant
2.4. Chlamydomonas MAPKK Family
2.5. Chlamydomonas MAPKK Gene Expression
2.6. Chlamydomonas MAPKKK Family
2.7. Gene Expression of MAPKKK Candidates to be Involved in the N Assimilation
3. Discussion
4. Materials and Methods
4.1. Chlamydomonas Strains and Growth Conditions
4.2. Chlamydomonas Genetic Cross
4.3. Nitrate Reductase Activity
4.4. Quantification of Chlamydomonas Growth
4.5. Quantification of Gene Expression by RT-qPCR
4.6. MAPK Cascade Identification
4.7. Phylogenetic and Domain Analysis
4.8. Additional Bioinformatics Tools
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gomez-Osuna, A.; Calatrava, V.; Galvan, A.; Fernandez, E.; Llamas, A. Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green Alga Chlamydomonas reinhardtii. Int. J. Mol. Sci. 2020, 21, 3417. https://doi.org/10.3390/ijms21103417
Gomez-Osuna A, Calatrava V, Galvan A, Fernandez E, Llamas A. Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green Alga Chlamydomonas reinhardtii. International Journal of Molecular Sciences. 2020; 21(10):3417. https://doi.org/10.3390/ijms21103417
Chicago/Turabian StyleGomez-Osuna, Aitor, Victoria Calatrava, Aurora Galvan, Emilio Fernandez, and Angel Llamas. 2020. "Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green Alga Chlamydomonas reinhardtii" International Journal of Molecular Sciences 21, no. 10: 3417. https://doi.org/10.3390/ijms21103417