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Open AccessArticle

Synthetic Human β Defensin-3-C15 Peptide in Endodontics: Potential Therapeutic Agent in Streptococcus gordonii Lipoprotein-Stimulated Human Dental Pulp-Derived Cells

1
Department of Conservative Dentistry, School of Dentistry, Dental Research Institute, Seoul National University, Daehak-ro 101, Chongno-Ku, Seoul 03080, Korea
2
Department of Dentistry, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON N6A 5C1, Canada
3
Central Research Institute, Nano intelligent Biomedical Engineering Corporation (NIBEC), Seoul 03130, Korea
4
Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul 02447, Korea
5
Department of Conservative Dentistry, School of Stomatology, Shandong University, Jinan 250012, China
6
Department of Oral Microbiology and Immunology, School of Dentistry, Dental Research Institute and BK21 Plus Program, Seoul National University, Seoul 08826, Korea
7
National Dental Care Center for Persons with Special Cares, Seoul National University Dental Hospital for Persons with Special Needs, Seoul 03080, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 71; https://doi.org/10.3390/ijms21010071
Received: 25 October 2019 / Revised: 16 December 2019 / Accepted: 17 December 2019 / Published: 20 December 2019
(This article belongs to the Special Issue Membrane–Peptide Interactions: From Basics to Current Applications)
Human β defensin-3-C15, an epithelium-derived cationic peptide that has antibacterial/antifungal and immuno-regulatory properties, is getting attention as potential therapeutic agent in endodontics. This study aimed to investigate if synthetic human β defensin-3-C15 (HBD3-C15) peptides could inhibit inflammatory responses in human dental pulp cells (hDPCs), which had been induced by gram-positive endodontic pathogen. hDPC explant cultures were stimulated with Streptococcus gordonii lipoprotein extracts for 24 h to induce expression of pro-inflammatory mediators. The cells were then treated with either HBD3-C15 (50 μg/mL) or calcium hydroxide (CH, 100 μg/mL) as control for seven days, to assess their anti-inflammatory effects. Quantitative RT-PCR analyses and multiplex assays showed that S. gordonii lipoprotein induced the inflammatory reaction in hDPCs. There was a significant reduction of IL-8 and MCP-1 within 24 h of treatment with either CH or HBD3-C15 (p < 0.05), which was sustained over 1 week of treatment. Alleviation of inflammation in both medications was related to COX-2 expression and PGE2 secretion (p < 0.05), rather than TLR2 changes (p > 0.05). These findings demonstrate comparable effects of CH and HDB3-C15 as therapeutic agents for inflamed hDPCs. View Full-Text
Keywords: calcium hydroxide; chemokine; human beta defensin-3-C15; human dental pulp cell; Streptococcus gordonii lipoprotein calcium hydroxide; chemokine; human beta defensin-3-C15; human dental pulp cell; Streptococcus gordonii lipoprotein
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Yoo, Y.-J.; Perinpanayagam, H.; Lee, J.-Y.; Oh, S.; Gu, Y.; Kim, A.-R.; Chang, S.-W.; Baek, S.-H.; Kum, K.-Y. Synthetic Human β Defensin-3-C15 Peptide in Endodontics: Potential Therapeutic Agent in Streptococcus gordonii Lipoprotein-Stimulated Human Dental Pulp-Derived Cells. Int. J. Mol. Sci. 2020, 21, 71.

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