Next Article in Journal
Effects of Regorafenib, a Multi-Kinase Inhibitor, on Conjunctival Scarring in a Canine Filtration Surgery Model in Comparison with Mitomycin-C
Next Article in Special Issue
Non-Coding RNAs Participate in the Regulation of CRY-DASH in the Growth and Early Development of Saccharina japonica (Laminariales, Phaeophyceae)
Previous Article in Journal
A Clinical Approach for the Use of VIP Axis in Inflammatory and Autoimmune Diseases
Previous Article in Special Issue
Rapid Molecular Identification of Scolytinae (Coleoptera: Curculionidae)
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 21
Issue 1
10.3390/ijms21010064
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri

by
Hai-Zhong Yu
1,2,†,
Ning-Yan Li
1,†,
Bing Li
3,†,
Shahzad Toufeeq
4,
Yan-Xin Xie
1,
Yu-Ling Huang
1,
Yi-Ming Du
1,2,
Xiang-Dong Zeng
1,
Bo Zhu
1,2 and
Zhan-Jun Lu
1,2,*
1
School of Life Sciences, Gannan Normal University, Ganzhou 341000, China
2
National Navel Orange Engineering and Technology Research Center, Gannan Normal University, Ganzhou 341000, China
3
Institute of Sericulture, Anhui Academy of Agricultural Sciences, Hefei 230031, China
4
School of Life Science, Anhui Agricultural University, Hefei 230031, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(1), 64; https://doi.org/10.3390/ijms21010064
Submission received: 1 November 2019 / Revised: 17 December 2019 / Accepted: 18 December 2019 / Published: 20 December 2019
(This article belongs to the Special Issue Molecular Ecology, Physiology and Biochemistry of Insects)
Browse Figures
Review Reports Versions Notes

Abstract

Chitin deacetylase (CDA) is a chitin degradation enzyme that strictly catalyzes the deacetylation of chitin to form chitosan, which plays an important role in regulating growth and development, as well as the immune response. In this study, a chitin deacetylase 3 gene (CDA3) was identified with a complete open reading frame (ORF) of 1362 bp from the genome database of Diaphorina citri, encoding a protein of 453 amino acids. Spatiotemporal expression analysis suggested that D. citri CDA3 (DcCDA3) had the highest expression level in the integument and third-instar nymph stage. Furthermore, DcCDA3 expression level can be induced by 20-hydroxyecdysone (20E). Injection of Escherichia coli and Staphylococcus aureus induced the upregulation of DcCDA3 in the midgut, while DcCDA3 was downregulated in the fat body. After silencing DcCDA3 by RNA interference, there was no influence on the D. citri phenotype. In addition, bactericidal tests showed that recombinant DcCDA3 inhibited gram-positive bacteria, including S. aureus and Bacillus subtilis (B. subtilis). In conclusion, our results suggest that DcCDA3 might play an important role in the immune response of D. citri.
Keywords: Diaphorina citri; chitin deacetylase 3; expression profiles; immune response Diaphorina citri; chitin deacetylase 3; expression profiles; immune response
Graphical Abstract

Share and Cite

MDPI and ACS Style

Yu, H.-Z.; Li, N.-Y.; Li, B.; Toufeeq, S.; Xie, Y.-X.; Huang, Y.-L.; Du, Y.-M.; Zeng, X.-D.; Zhu, B.; Lu, Z.-J. Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri. Int. J. Mol. Sci. 2020, 21, 64. https://doi.org/10.3390/ijms21010064

AMA Style

Yu H-Z, Li N-Y, Li B, Toufeeq S, Xie Y-X, Huang Y-L, Du Y-M, Zeng X-D, Zhu B, Lu Z-J. Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri. International Journal of Molecular Sciences. 2020; 21(1):64. https://doi.org/10.3390/ijms21010064

Chicago/Turabian Style

Yu, Hai-Zhong, Ning-Yan Li, Bing Li, Shahzad Toufeeq, Yan-Xin Xie, Yu-Ling Huang, Yi-Ming Du, Xiang-Dong Zeng, Bo Zhu, and Zhan-Jun Lu. 2020. "Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri" International Journal of Molecular Sciences 21, no. 1: 64. https://doi.org/10.3390/ijms21010064

APA Style

Yu, H.-Z., Li, N.-Y., Li, B., Toufeeq, S., Xie, Y.-X., Huang, Y.-L., Du, Y.-M., Zeng, X.-D., Zhu, B., & Lu, Z.-J. (2020). Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri. International Journal of Molecular Sciences, 21(1), 64. https://doi.org/10.3390/ijms21010064

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop