Next Article in Journal
Characterization of Effectiveness in Concerted Ih Inhibition and IK(Ca) Stimulation by Pterostilbene (Trans-3,5-dimethoxy-4′-hydroxystilbene), a Stilbenoid
Next Article in Special Issue
Effect of F-actin and Microtubules on Cellular Mechanical Behavior Studied Using Atomic Force Microscope and an Image Recognition-Based Cytoskeleton Quantification Approach
Previous Article in Journal
miR-218 Inhibits Mitochondrial Clearance by Targeting PRKN E3 Ubiquitin Ligase
Previous Article in Special Issue
Arabidopsis Class II Formins AtFH13 and AtFH14 Can Form Heterodimers but Exhibit Distinct Patterns of Cellular Localization
Open AccessArticle

Coronin 1 Is Required for Integrin β2 Translocation in Platelets

1
Centre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK
2
School of Physiology, Pharmacology and Neuroscience, Faculty of Life Sciences, University of Bristol, Bristol BS8 1TD, UK
3
Biozentrum, University of Basel, CH-4056 Basel, Switzerland
4
Leeds Institute for Cardiovascular and Metabolic Medicine, University of Leeds, Leeds LS2 9NL, UK
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 356; https://doi.org/10.3390/ijms21010356
Received: 22 November 2019 / Revised: 23 December 2019 / Accepted: 1 January 2020 / Published: 5 January 2020
(This article belongs to the Special Issue Frontiers in the Actin Cytoskeleton)
Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the actin cytoskeleton, represented by the abundant coronins 1, 2, and 3 and the less abundant coronin 7 in platelets, but their functions in these cells are poorly understood. A recent report revealed impaired agonist-induced actin polymerization and cofilin phosphoregulation and altered thrombus formation in vivo as salient phenotypes in the absence of an overt hemostasis defect in vivo in a knockout mouse model of coronin 1. Here we show that the absence of coronin 1 is associated with impaired translocation of integrin β2 to the platelet surface upon stimulation with thrombin while morphological and functional alterations, including defects in Arp2/3 complex localization and cAMP-dependent signaling, are absent. Our results suggest a large extent of functional overlap among coronins 1, 2, and 3 in platelets, while aspects like integrin β2 translocation are specifically or predominantly dependent on coronin 1. View Full-Text
Keywords: actin; Arp2/3 complex; cAMP; coronin 1; integrin β2; platelets; thrombin; collagen; prostacyclin actin; Arp2/3 complex; cAMP; coronin 1; integrin β2; platelets; thrombin; collagen; prostacyclin
Show Figures

Figure 1

MDPI and ACS Style

Riley, D.R.J.; Khalil, J.S.; Pieters, J.; Naseem, K.M.; Rivero, F. Coronin 1 Is Required for Integrin β2 Translocation in Platelets. Int. J. Mol. Sci. 2020, 21, 356.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop