Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii
Abstract
:1. Introduction
2. Results
2.1. RNA-seq Quantity, Sequence Assembly, and Differential Expression Gene (DEGs) Analysis
2.2. Validation of DEGs by Quantitative RT-PCR (qRT-PCR)
2.3. Chromosome Fraction Length Interval Assignment of Chromosome 2Ss#1-Specific Markers
2.4. Development of CS-Ae. searsii Disomic 2Ss#1 Recombinants and Physical Mapping of Pm57
2.5. Comparative Synteny and Genes in Pm57 Candidate Region
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Construction of cDNA Libraries for Illumina Sequencing
4.3. RNA-seq Data Analysis
4.4. Validations of RNA-seq Data by Quantitative RT-PCR
4.5. Molecular Marker Analysis
4.6. Powdery Mildew Response Assay
4.7. Identification of CS-Ae. searsii 2Ss#1 Recombinants and Physical Mapping of Pm57
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
Bgt | Blumeria graminis f. sp. tritici |
FL | fraction length |
CNL | coiled-coil nucleotide-binding site-leucine-rich repeat |
DEG | differential expression gene |
SFL | short-arm fraction length |
LFL | long-arm fraction length |
RPKM | reads per kilobase per million mapped reads |
IT | infection types |
VIGS | virus-induced gene silencing |
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WGRC 1 Accession Number | Description | Fraction Length Interval Harboring 2Ss#1 Segment | Reference |
---|---|---|---|
TA3808 | CS | - 2 | - 3 |
TA3809 | CS ph1b mutant | - 2 | Sears (1977) [50] |
TA3581 (2011-400) | CS-Ae. searsii disomic 2Ss#1 addition line | - 2 | Friebe et al. (1995) [51] |
TA5109 (89-69) | CS-Ae. searsii T2BS.2BL-2Ss#1L recombinant line | LFL0.67-1.00 | Liu et al. (2017) [7] |
89-88 | CS-Ae. searsii Ti2AS-2Ss#1S.2Ss#1L-2AL recombinant line | SFL0.70-LFL0.87 | Liu et al. (2017) [7] |
89-152 4 | CS-Ae. searsii TiW?S-2Ss#1S-W?S.W?L recombinant line | SFL0.35-0.82 | Liu et al. (2017) [7] |
89-185 | CS-Ae. searsii ditelosomic 2Ss#1L addition line | LFLC-1.00 | Liu et al. (2017) [7] |
89-346 | CS-Ae. searsii T2BS.2BL-2Ss#1L recombinant line | LFL0.72-1.00 | Liu et al. (2017) [7] |
89-378 4 | CS-Ae. searsii T2Ss#1S.2Ss#1L-2W? recombinant line | SFL1.00-LFL0.64 | Liu et al. (2017) [7] |
Locus 1 No. | Marker Name | Chromosome Bin | Genomic Position (Mb) 2 |
---|---|---|---|
1 | X23241 | LFLC-0.64 | 488.87 |
2 | X216815 | LFC0.64-0.67 | 658.61 |
3 | X170551 | LFL0.72-0.87 | 320.50 |
4 | X123945 | LFL0.72-0.87 | 622.52 |
5 | X185442 | LFL0.72-0.87 | 733.47 |
6 | X305960 | LFL0.72-0.87 | 744.52 |
7 | X26866 | LFL0.72-0.87 | 752.95 |
8 | X284274 | LFL0.72-0.87 | 748.97 |
9 | X37693 | LFL0.72-0.87 | 754.92 |
10 | X251565 | LFL0.72-0.87 | 777.64 |
11 | X103528 | LFL0.72-0.87 | 762.50 |
12 | X5286/X315363 | LFL0.72-0.87 | 765.05 |
13 | X33739 | LFL0.72-0.87 | 766.23 |
14 | X268081 | LFL0.72-0.87 | 768.61 |
15 | X49873 | LFL0.72-0.87 | 770.18 |
16 | X256410 | LFL0.72-0.87 | 771.04 |
17 | X67593 | LFL0.72-0.87 | 773.72 |
18 | X33705 | LFL0.72-0.87 | 775.02 |
19 | X430422/X285619/X205834/X2407243 | LFL0.72-0.87 | 775.33 |
20 | X4364 | LFL0.72-0.87 | 778.33 |
21 | X62492 | LFL0.72-0.87 | 778.85 |
22 | X55871 | LFL0.72-0.87 | 779.34 |
23 | X23212 | LFL0.87-1.00 | 783.60 |
24 | X2L4g9p4 | LFL0.87-1.00 | 788.66 |
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Dong, Z.; Tian, X.; Ma, C.; Xia, Q.; Wang, B.; Chen, Q.; Sehgal, S.K.; Friebe, B.; Li, H.; Liu, W. Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii. Int. J. Mol. Sci. 2020, 21, 322. https://doi.org/10.3390/ijms21010322
Dong Z, Tian X, Ma C, Xia Q, Wang B, Chen Q, Sehgal SK, Friebe B, Li H, Liu W. Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii. International Journal of Molecular Sciences. 2020; 21(1):322. https://doi.org/10.3390/ijms21010322
Chicago/Turabian StyleDong, Zhenjie, Xiubin Tian, Chao Ma, Qing Xia, Beilin Wang, Qifan Chen, Sunish K. Sehgal, Bernd Friebe, Huanhuan Li, and Wenxuan Liu. 2020. "Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii" International Journal of Molecular Sciences 21, no. 1: 322. https://doi.org/10.3390/ijms21010322