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Article

Expression of Musashi-1 During Osteogenic Differentiation of Oral MSC: An In Vitro Study

1
Department of Oral Surgery and Implant Dentistry. School of Dentistry, University of Granada, Granada 18071, Spain
2
Servicio de Analisis Clinicos e Inmunologia, UGC Laboratorio Clinico, Hospital Universitario Virgen de las Nieves, Granada 18014, Spain
3
Biosanitary Institute of Granada (ibs.Granada). University of Granada, Granada 18071, Spain
4
Department of Pathology and IBIMER. School of Medicine, University of Granada, Granada 18071, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(9), 2171; https://doi.org/10.3390/ijms20092171
Received: 26 March 2019 / Revised: 26 April 2019 / Accepted: 29 April 2019 / Published: 2 May 2019
(This article belongs to the Special Issue Role and Application of Stem Cells in Regenerative Medicine)
Background: Musashi-1 (MSI1) is a negative regulator of mesenchymal stromal cell (MSC) differentiation which in turn favors cell proliferation. However, little is known about its expression by MSC from the oral cavity and in the context of osteogenic differentiation. Aim: The aim of this study was to analyze the expression of MSI1 in the context of osteogenic differentiation of MSC derived from the oral cavity. Material/methods: For this in vitro study, MSC were isolated from six different origins of the oral cavity. They were extensively characterized in terms of proliferative and clonogenicity potential, expression of stemness genes (MYC, NANOG, POU5F1, and SOX2), expression of surface markers (CD73, CD90, CD105, CD14, CD31, CD34, and CD45) and adipo-, chondro- and osteogenic differentiation potential. Then, osteogenic differentiation was induced and the expression of MSI1 mRNA and other relevant markers of osteogenic differentiation, including RUNX2 and Periostin, were also evaluated. Results: Cell populations from the alveolar bone (pristine or previously grafted with xenograft), dental follicle, dental germ, dental pulp, and periodontal ligament were obtained. The analysis of proliferative and clonogenicity potential, expression of the stemness genes, expression of surface markers, and differentiation potential showed similar characteristics to those of previously published MSC from the umbilical cord. Under osteogenic differentiation conditions, all MSC populations formed calcium deposits and expressed higher SPARC. Over time, the expression of MSI1 followed different patterns for the different MSC populations. It was not significantly different than the expression of RUNX2. In contrast, the expression of MSI1 and POSTN and RUNX2 were statistically different in most MSC populations. Conclusion: In the current study, a similar expression pattern of MSI1 and RUNX2 during in vitro osteogenic differentiation was identified. View Full-Text
Keywords: musashi-1; periostin; RUNX2; mesenchymal stromal cells; osteogenic differentiation; bone regeneration; bone healing musashi-1; periostin; RUNX2; mesenchymal stromal cells; osteogenic differentiation; bone regeneration; bone healing
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MDPI and ACS Style

Padial-Molina, M.; de Buitrago, J.G.; Sainz-Urruela, R.; Abril-Garcia, D.; Anderson, P.; O’Valle, F.; Galindo-Moreno, P. Expression of Musashi-1 During Osteogenic Differentiation of Oral MSC: An In Vitro Study. Int. J. Mol. Sci. 2019, 20, 2171. https://doi.org/10.3390/ijms20092171

AMA Style

Padial-Molina M, de Buitrago JG, Sainz-Urruela R, Abril-Garcia D, Anderson P, O’Valle F, Galindo-Moreno P. Expression of Musashi-1 During Osteogenic Differentiation of Oral MSC: An In Vitro Study. International Journal of Molecular Sciences. 2019; 20(9):2171. https://doi.org/10.3390/ijms20092171

Chicago/Turabian Style

Padial-Molina, Miguel, Juan G. de Buitrago, Raquel Sainz-Urruela, Dario Abril-Garcia, Per Anderson, Francisco O’Valle, and Pablo Galindo-Moreno. 2019. "Expression of Musashi-1 During Osteogenic Differentiation of Oral MSC: An In Vitro Study" International Journal of Molecular Sciences 20, no. 9: 2171. https://doi.org/10.3390/ijms20092171

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