Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen

Štambuk, Nikola; Konjevoda, Paško; Turčić, Petra; Šošić, Hrvoje; Aralica, Gorana; Babić, Damir; Seiwerth, Sven; Kaštelan, Željko; Kujundžić, Renata Novak; Wardega, Piotr; Žutelija, Jelena Barać; Gračanin, Ana Gudelj; Gabričević, Mario

doi:10.3390/ijms20092090

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Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen

^1,*,†

^2,†

^3,*

⁴,

^5,6,

^6,7,

^6,7,

⁸,

Renata Novak Kujundžić

²,

Piotr Wardega

⁹,

Jelena Barać Žutelija

¹⁰,

Ana Gudelj Gračanin

¹¹ and

Mario Gabričević

¹²

Center for Nuclear Magnetic Resonance, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia

Laboratory for Epigenomics, Division of Molecular Medicine, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia

Department of Pharmacology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Domagojeva 2, HR-10000 Zagreb, Croatia

⁴

Department of Urology, Clinical Hospital Centre Split, Šoltanska 1, HR-21000 Split, Croatia

⁵

University Hospital Dubrava, Avenija Gojka Šuška 6, HR-10000 Zagreb, Croatia

⁶

Department of Pathology, School of Medicine, University of Zagreb, Šalata 10, HR-10000 Zagreb, Croatia

⁷

Department of Pathology, University Hospital Centre Zagreb, Kišpatićeva 12, HR-10000 Zagreb, Croatia

⁸

Department of Urology, University Hospital Centre Zagreb, Kišpatićeva 12, HR-10000 Zagreb, Croatia

⁹

NanoTemper Technologies sp. z o.o., Bobrzynskiego 14, 30-348 Krakow, Poland

¹⁰

Clincal Department of Pathology “Ljudevit Jurak”, Clinical Hospital Center “Sestre milosrdnice”, Vinogradska 29, 10000 Zagreb, Croatia

¹¹

Department of Internal Medicine, Division of Clinical Immunology, Allergology and Rheumatology, School of Medicine, University of Zagreb, Dubrava University Hospital, Avenija Gojka Šuška 6, HR-10000 Zagreb, Croatia

¹²

Department of General and Inorganic Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, HR-10000 Zagreb, Croatia

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^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2019, 20(9), 2090; https://doi.org/10.3390/ijms20092090

Received: 24 January 2019 / Revised: 5 April 2019 / Accepted: 25 April 2019 / Published: 28 April 2019

(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)

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Abstract

The purpose of this paper was to outline the development of short peptide targeting of the human prostate specific antigen (hPSA), and to evaluate its effectiveness in staining PSA in human prostate cancer tissue. The targeting of the hPSA antigen by means of antisense peptide AVRDKVG was designed according to a three-step method involving: 1. The selection of the molecular target (hPSA epitope), 2. the modeling of an antisense peptide (paratope) based on the epitope sequence, and 3. the spectroscopic evaluation of sense–antisense peptide binding. We then modified standard hPSA immunohistochemical staining practice by using a biotinylated antisense peptide instead of the standard monoclonal antibody and compared the results of both procedures. Immunochemical testing on human tissue showed the applicability of the antisense peptide technology to human molecular targets. This methodology represents a new approach to deriving peptide ligands and potential lead compounds for the development of novel diagnostic substances, biopharmaceuticals and vaccines. View Full-Text

Keywords: neoplasm; prostate specific antigen; biomarker; antisense peptide; immunohistochemistry; nanomedicine

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MDPI and ACS Style

Štambuk, N.; Konjevoda, P.; Turčić, P.; Šošić, H.; Aralica, G.; Babić, D.; Seiwerth, S.; Kaštelan, Ž.; Kujundžić, R.N.; Wardega, P.; Žutelija, J.B.; Gračanin, A.G.; Gabričević, M. Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen. Int. J. Mol. Sci. 2019, 20, 2090. https://doi.org/10.3390/ijms20092090

AMA Style

Štambuk N, Konjevoda P, Turčić P, Šošić H, Aralica G, Babić D, Seiwerth S, Kaštelan Ž, Kujundžić RN, Wardega P, Žutelija JB, Gračanin AG, Gabričević M. Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen. International Journal of Molecular Sciences. 2019; 20(9):2090. https://doi.org/10.3390/ijms20092090

Chicago/Turabian Style

Štambuk, Nikola, Paško Konjevoda, Petra Turčić, Hrvoje Šošić, Gorana Aralica, Damir Babić, Sven Seiwerth, Željko Kaštelan, Renata Novak Kujundžić, Piotr Wardega, Jelena Barać Žutelija, Ana Gudelj Gračanin, and Mario Gabričević. 2019. "Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen" International Journal of Molecular Sciences 20, no. 9: 2090. https://doi.org/10.3390/ijms20092090

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