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Int. J. Mol. Sci. 2019, 20(8), 1863; https://doi.org/10.3390/ijms20081863

Deep Transcriptome Analysis Reveals Reactive Oxygen Species (ROS) Network Evolution, Response to Abiotic Stress, and Regulation of Fiber Development in Cotton

1
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences (ICR, CAAS), Anyang 455000, China
2
Jaramogi Oginga Odinga University of Science and Technology (JOOUST), School of Biological and Physical Sciences (SPBS), P.O BOX 210-40600, Bondo 210-40600, Kenya
3
Biological and Food Engineering, Anyang Institute of Technology, Anyang 455000, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 28 February 2019 / Revised: 8 April 2019 / Accepted: 8 April 2019 / Published: 15 April 2019
(This article belongs to the Special Issue Mapping Abiotic Stress-Tolerance Genes in Plants)
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Abstract

Reactive oxygen species (ROS) are important molecules in the plant, which are involved in many biological processes, including fiber development and adaptation to abiotic stress in cotton. We carried out transcription analysis to determine the evolution of the ROS genes and analyzed their expression levels in various tissues of cotton plant under abiotic stress conditions. There were 515, 260, and 261 genes of ROS network that were identified in Gossypium hirsutum (AD1 genome), G. arboreum (A genome), and G. raimondii (D genome), respectively. The ROS network genes were found to be distributed in all the cotton chromosomes, but with a tendency of aggregating on either the lower or upper arms of the chromosomes. Moreover, all the cotton ROS network genes were grouped into 17 families as per the phylogenetic tress analysis. A total of 243 gene pairs were orthologous in G. arboreum and G. raimondii. There were 240 gene pairs that were orthologous in G. arboreum, G. raimondii, and G. hirsutum. The synonymous substitution value (Ks) peaks of orthologous gene pairs between the At subgenome and the A progenitor genome (G. arboreum), D subgenome and D progenitor genome (G. raimondii) were 0.004 and 0.015, respectively. The Ks peaks of ROS network orthologous gene pairs between the two progenitor genomes (A and D genomes) and two subgenomes (At and Dt subgenome) were 0.045. The majority of Ka/Ks value of orthologous gene pairs between the A, D genomes and two subgenomes of TM-1 were lower than 1.0. RNA seq. analysis and RT-qPCR validation, showed that, CSD1,2,3,5,6; FSD1,2; MSD1,2; APX3,11; FRO5.6; and RBOH6 played a major role in fiber development while CSD1, APX1, APX2, MDAR1, GPX4-6-7, FER2, RBOH6, RBOH11, and FRO5 were integral for enhancing salt stress in cotton. ROS network-mediated signal pathway enhances the mechanism of fiber development and regulation of abiotic stress in Gossypium. This study will enhance the understanding of ROS network and form the basic foundation in exploring the mechanism of ROS network-involving the fiber development and regulation of abiotic stress in cotton. View Full-Text
Keywords: ROS genes; transcription analysis; abiotic stress; fiber development ROS genes; transcription analysis; abiotic stress; fiber development
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Xu, Y.; Magwanga, R.O.; Cai, X.; Zhou, Z.; Wang, X.; Wang, Y.; Zhang, Z.; Jin, D.; Guo, X.; Wei, Y.; Li, Z.; Wang, K.; Liu, F. Deep Transcriptome Analysis Reveals Reactive Oxygen Species (ROS) Network Evolution, Response to Abiotic Stress, and Regulation of Fiber Development in Cotton. Int. J. Mol. Sci. 2019, 20, 1863.

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