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Open AccessArticle

HvPAA1 Encodes a P-Type ATPase, a Novel Gene for Cadmium Accumulation and Tolerance in Barley (Hordeum vulgare L.)

1
Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China
2
School of Agriculture, Food and Wine, the University of Adelaide, Waite Campus, Adelaide 5064, Australia
3
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(7), 1732; https://doi.org/10.3390/ijms20071732
Received: 1 March 2019 / Revised: 1 April 2019 / Accepted: 2 April 2019 / Published: 8 April 2019
(This article belongs to the Special Issue Mapping Abiotic Stress-Tolerance Genes in Plants)
The identification of gene(s) that are involved in Cd accumulation/tolerance is vital in developing crop cultivars with low Cd accumulation. We developed a doubled haploid (DH) population that was derived from a cross of Suyinmai 2 (Cd-sensitive) × Weisuobuzhi (Cd-tolerant) to conduct quantitative trait loci (QTL) mapping studies. We assessed chlorophyll content, traits that are associated with development, metal concentration, and antioxidative enzyme activity in DH population lines and parents under control and Cd stress conditions. A single QTL, designated as qShCd7H, was identified on chromosome 7H that was linked to shoot Cd concentration; qShCd7H explained 17% of the phenotypic variation. Comparative genomics, map-based cloning, and gene silencing were used in isolation, cloning, and functional characterization of the candidate gene. A novel gene HvPAA1, being related to shoot Cd concentration, was identified from qShCd7H. Sequence comparison indicated that HvPAA1 carried seven domains with an N-glycosylation motif. HvPAA1 is predominantly expressed in shoots. Subcellular localization verified that HvPAA1 is located in plasma membrane. The silencing of HvPAA1 resulted in growth inhibition, greater Cd accumulation, and a significant decrease in Cd tolerance. We conclude HvPAA1 is a novel plasma membrane-localized ATPase that contributes to Cd tolerance and accumulation in barley. The results provide us with new insights that may aid in the screening and development of Cd-tolerant and low-Cd-accumulation crops. View Full-Text
Keywords: barley (Hordeum vulgare L.); BSMV-VIGS; cadmium tolerance and accumulation; HvPAA1; quantitative trait loci (QTLs) barley (Hordeum vulgare L.); BSMV-VIGS; cadmium tolerance and accumulation; HvPAA1; quantitative trait loci (QTLs)
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MDPI and ACS Style

Wang, X.-K.; Gong, X.; Cao, F.; Wang, Y.; Zhang, G.; Wu, F. HvPAA1 Encodes a P-Type ATPase, a Novel Gene for Cadmium Accumulation and Tolerance in Barley (Hordeum vulgare L.). Int. J. Mol. Sci. 2019, 20, 1732.

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