CUBAN, a Case Study of Selective Binding: Structural Details of the Discrimination between Ubiquitin and NEDD8
Abstract
:1. Introduction
2. Results
2.1. Interaction of the 15N Labeled CUBAN Domain with NEDD8 and Ubiquitin
2.2. Interaction of 15N Labeled NEDD8 with the CUBAN Domain
2.3. Structural Dynamics of the CUBAN Domain in Complex with NEDD8
3. Discussion
Conformational Selection or Induced Fit?
4. Materials and Methods
4.1. Expression Plasmids and Protein Purification
4.2. NMR Spectroscopy
4.3. Molecular Dynamics Simulations
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Santonico, E.; Nepravishta, R.; Mandaliti, W.; Castagnoli, L.; Cesareni, G.; Paci, M. CUBAN, a Case Study of Selective Binding: Structural Details of the Discrimination between Ubiquitin and NEDD8. Int. J. Mol. Sci. 2019, 20, 1185. https://doi.org/10.3390/ijms20051185
Santonico E, Nepravishta R, Mandaliti W, Castagnoli L, Cesareni G, Paci M. CUBAN, a Case Study of Selective Binding: Structural Details of the Discrimination between Ubiquitin and NEDD8. International Journal of Molecular Sciences. 2019; 20(5):1185. https://doi.org/10.3390/ijms20051185
Chicago/Turabian StyleSantonico, Elena, Ridvan Nepravishta, Walter Mandaliti, Luisa Castagnoli, Gianni Cesareni, and Maurizio Paci. 2019. "CUBAN, a Case Study of Selective Binding: Structural Details of the Discrimination between Ubiquitin and NEDD8" International Journal of Molecular Sciences 20, no. 5: 1185. https://doi.org/10.3390/ijms20051185