Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling

Tang, Pei; Wang, Xueer; Zhang, Min; Huang, Simin; Lin, Chuxi; Yan, Fang; Deng, Ying; Zhang, Lu; Zhang, Lin

doi:10.3390/ijms20040853

Open AccessArticle

Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling

by

Pei Tang

^1,†,

Xueer Wang

^1,†,

Min Zhang

¹,

Simin Huang

¹,

Chuxi Lin

¹,

Fang Yan

¹,

Ying Deng

¹,

Lu Zhang

^2,*

and

Lin Zhang

^1,*

¹

Guangdong Provincial Key Laboratory of Tissue Construction and Detection, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China

²

Guangdong Provincial Key Laboratory of Proteomics, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2019, 20(4), 853; https://doi.org/10.3390/ijms20040853

Submission received: 24 December 2018 / Revised: 5 February 2019 / Accepted: 9 February 2019 / Published: 15 February 2019

(This article belongs to the Special Issue MAPK-ERK Pathway)

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Abstract

Activins and their receptors play important roles in the control of hair follicle morphogenesis, but their role in vibrissae follicle growth remains unclear. To investigate the effect of Activin B on vibrissae follicles, the anagen induction assay and an in vitro vibrissae culture system were constructed. Hematoxylin and eosin staining were performed to determine the hair cycle stages. The 5-ethynyl-2′-deoxyuridine (EdU) and Cell Counting Kit-8 (CCK-8) assays were used to examine the cell proliferation. Flow cytometry was used to detect the cell cycle phase. Inhibitors and Western blot analysis were used to investigate the signaling pathway induced by Activin B. As a result, we found that the vibrissae follicle growth was accelerated by 10 ng/mL Activin B in the anagen induction assay and in an organ culture model. 10 ng/mL Activin B promoted hair matrix cell proliferation in vivo and in vitro. Moreover, Activin B modulates hair matrix cell growth through the ERK–Elk1 signaling pathway, and Activin B accelerates hair matrix cell transition from the G1/G0 phase to the S phase through the ERK–Cyclin D1 signaling pathway. Taken together, these results demonstrated that Activin B may promote mouse vibrissae growth by stimulating hair matrix cell proliferation and cell cycle progression through ERK signaling.

Keywords: activin; vibrissae; hair matrix cell; cell proliferation; ERK

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MDPI and ACS Style

Tang, P.; Wang, X.; Zhang, M.; Huang, S.; Lin, C.; Yan, F.; Deng, Y.; Zhang, L.; Zhang, L. Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling. Int. J. Mol. Sci. 2019, 20, 853. https://doi.org/10.3390/ijms20040853

AMA Style

Tang P, Wang X, Zhang M, Huang S, Lin C, Yan F, Deng Y, Zhang L, Zhang L. Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling. International Journal of Molecular Sciences. 2019; 20(4):853. https://doi.org/10.3390/ijms20040853

Chicago/Turabian Style

Tang, Pei, Xueer Wang, Min Zhang, Simin Huang, Chuxi Lin, Fang Yan, Ying Deng, Lu Zhang, and Lin Zhang. 2019. "Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling" International Journal of Molecular Sciences 20, no. 4: 853. https://doi.org/10.3390/ijms20040853

APA Style

Tang, P., Wang, X., Zhang, M., Huang, S., Lin, C., Yan, F., Deng, Y., Zhang, L., & Zhang, L. (2019). Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling. International Journal of Molecular Sciences, 20(4), 853. https://doi.org/10.3390/ijms20040853

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Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling

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