Protective Effect of Antrodia cinnamomea Extract against Irradiation-Induced Acute Hepatitis
Abstract
:1. Introduction
2. Results
2.1. Radio-Protective Effects of Antrodia cinnamomea Extracts in Liver Cells
2.2. ACE Protects Liver Cells from Irradiation-Induced Apoptosis
2.3. Evaluation of Direct Free Radical-Scavenging Activity of ACE in Solution
2.4. Irradiation-Induced ROS Scavenging Activity of ACE in Liver Cells
2.5. ACE Enhances the Irradiation-Induced Redox Enzymes Activities of Hepatocytes
2.6. ACE Enhances the Expression and Nuclear Translocation of Nrf2 in Irradiated Hepatocytes
2.7. ACE Alleviates the Irradiation-Induced Liver Inflammation In Vivo
3. Discussion
4. Materials and Methods
4.1. Irradiation Treatment
4.2. Antrodia Cinnamomea from Fruiting Bodies in Cultivation Bags
4.3. Preparation of Ethanol Extracts of Antrodia cinnamomea
4.4. Cell Culture and Viability Assay
4.5. Apoptosis Analysis
4.6. Stable Free Radical Scavenging Activity Assay
4.7. Measurement of Intracellular Free Radicals
4.8. Measurement of Superoxide Dismutase and Glutathione Peroxidase Activities
4.9. Semi-quantitative RT-PCR and Western Blot Analysis
4.10. Immunofluorecscence Staining of Nrf2
4.11. Tumor Allografts and In Vitro Molecular Imaging
4.12. Immunohistochemical Staining
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
HCC | hepatocellular carcinoma |
RILD | radiation-induced liver disease |
ROS | reactive oxygen species |
AST | aspartate transaminase |
ALT | alanine transaminase |
AC | Antrodia cinnamomea |
ACE | Antrodia cinnamomea extract |
Nrf2 | Nuclear factor erythroid-2-related factor |
DCFH-DA | Theoxidation of 2′,7′-dichlorofluorescin diacetate |
ESR | electron spin resonance |
DAPI | 4′,6-diamidino-2-phenylindole |
IHC | Immunohistochemical |
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Kuo, T.-H.; Kuo, Y.-H.; Cho, C.-Y.; Yao, C.-J.; Lai, G.-M.; Chuang, S.-E. Protective Effect of Antrodia cinnamomea Extract against Irradiation-Induced Acute Hepatitis. Int. J. Mol. Sci. 2019, 20, 846. https://doi.org/10.3390/ijms20040846
Kuo T-H, Kuo Y-H, Cho C-Y, Yao C-J, Lai G-M, Chuang S-E. Protective Effect of Antrodia cinnamomea Extract against Irradiation-Induced Acute Hepatitis. International Journal of Molecular Sciences. 2019; 20(4):846. https://doi.org/10.3390/ijms20040846
Chicago/Turabian StyleKuo, Tsu-Hsiang, Yueh-Hsiung Kuo, Chun-Yu Cho, Chih-Jung Yao, Gi-Ming Lai, and Shuang-En Chuang. 2019. "Protective Effect of Antrodia cinnamomea Extract against Irradiation-Induced Acute Hepatitis" International Journal of Molecular Sciences 20, no. 4: 846. https://doi.org/10.3390/ijms20040846