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Open AccessArticle

Bioactive Polyphenols Modulate Enzymes Involved in Grapevine Pathogenesis and Chitinase Activity at Increasing Complexity Levels

1
Department of Agriculture, Food, Environmental and Animal Sciences, Plant Biology Unit, University of Udine, via delle Scienze 91, 33100 Udine, Italy
2
Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via Mach 1, 38010 San Michele all’Adige (TN), Italy
3
Department of Animal Biotechnology, Faculty of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161 Tehran, Iran
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(24), 6357; https://doi.org/10.3390/ijms20246357
Received: 17 November 2019 / Revised: 11 December 2019 / Accepted: 13 December 2019 / Published: 17 December 2019
(This article belongs to the Special Issue Plant Elicitors of Resistance and the Future of Plant Protection)
The reduction of synthetic chemistry use in modern viticulture relies on either the biological control of microorganisms or the induction of pathogenesis-related proteins. In the present study, the effects of hydro-alcoholic plant extracts (PEs) (i.e., by-products of Vitis vinifera L., leaves of Olea europaea L. and Ailanthus altissima (Mill.) Swingle) were tested on purified enzymes activity involved in plant-pathogen interactions. The polyphenolic composition was assayed and analyzed to characterize the extract profiles. In addition, suspension cell cultures of grapevine were treated with PEs to study their modulation of chitinase activity. Application of grape marc’s PE enhanced chitinase activity at 4 g L−1. Additionally, foliar treatment of grape marc’s PE at two doses (4 g L−1 and 800 g L−1) on grapevine cuttings induced a concentration-dependent stimulation of chitinase activity. The obtained results showed that the application of bioactive compounds based on PEs, rich in phenolic compounds, was effective both at in vitro and ex/in vivo level. The overall effects of PEs on plant-pathogen interaction were further discussed by applying a multi-criteria decision analysis, showing that grape marc was the most effective extract. View Full-Text
Keywords: Ailanthus altissima; chitinase; plant extracts; Olea europaea; pathogenesis-related protein; polyphenols; TOPSIS; Vitis vinifera Ailanthus altissima; chitinase; plant extracts; Olea europaea; pathogenesis-related protein; polyphenols; TOPSIS; Vitis vinifera
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Filippi, A.; Petrussa, E.; Boscutti, F.; Vuerich, M.; Vrhovsek, U.; Rabiei, Z.; Braidot, E. Bioactive Polyphenols Modulate Enzymes Involved in Grapevine Pathogenesis and Chitinase Activity at Increasing Complexity Levels. Int. J. Mol. Sci. 2019, 20, 6357.

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