Impact of Paraburkholderia phytofirmans PsJN on Grapevine Phenolic Metabolism
by
Lidiane Miotto-Vilanova 1,†, Barbara Courteaux 1,†, Rosa Padilla 2
, Fanja Rabenoelina 1, Cédric Jacquard 1, Christophe Clément 1, Gilles Comte 2, Céline Lavire 2, Essaïd Ait Barka 1, Isabelle Kerzaon 2 and Lisa Sanchez 1,*
Lidiane Miotto-Vilanova 1,†, Barbara Courteaux 1,†, Rosa Padilla 2, Fanja Rabenoelina 1, Cédric Jacquard 1, Christophe Clément 1, Gilles Comte 2, Céline Lavire 2, Essaïd Ait Barka 1, Isabelle Kerzaon 2 and Lisa Sanchez 1,*
1
Unité de Recherche EA 4707 Résistance Induite et Bioprotection des Plantes (RIBP), Université de Reims Champagne-Ardenne, SFR Condorcet FR CNRS 3417, 51687 Reims Cedex 2, France
2
Ecologie Microbienne, Université Lyon 1, CNRS, INRA, UMR 5557, 69622 Villeurbanne, France
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this paper.
Int. J. Mol. Sci. 2019, 20(22), 5775; https://doi.org/10.3390/ijms20225775
Received: 24 October 2019 / Revised: 12 November 2019 / Accepted: 13 November 2019 / Published: 16 November 2019
(This article belongs to the Special Issue Plant Elicitors of Resistance and the Future of Plant Protection)
Phenolic compounds are implied in plant-microorganisms interaction and may be induced in response to plant growth-promoting rhizobacteria (PGPRs). Among PGPR, the beneficial bacterium Paraburkholderia phytofirmans PsJN was previously described to stimulate the growth of plants and to induce a better adaptation to both abiotic and biotic stresses. This study aimed to investigate the impact of PsJN on grapevine secondary metabolism. For this purpose, gene expression (qRT-PCR) and profiling of plant secondary metabolites (UHPLC-UV/DAD-MS QTOF) from both grapevine root and leaves were compared between non-bacterized and PsJN-bacterized grapevine plantlets. Our results showed that PsJN induced locally (roots) and systemically (leaves) an overexpression of PAL and STS and specifically in leaves the overexpression of all the genes implied in phenylpropanoid and flavonoid pathways. Moreover, the metabolomic approach revealed that relative amounts of 32 and 17 compounds in roots and leaves, respectively, were significantly modified by PsJN. Once identified to be accumulated in response to PsJN by the metabolomic approach, antifungal properties of purified molecules were validated in vitro for their antifungal effect on Botrytis cinerea spore germination. Taking together, our findings on the impact of PsJN on phenolic metabolism allowed us to identify a supplementary biocontrol mechanism developed by this PGPR to induce plant resistance against pathogens.
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MDPI and ACS Style
Miotto-Vilanova, L.; Courteaux, B.; Padilla, R.; Rabenoelina, F.; Jacquard, C.; Clément, C.; Comte, G.; Lavire, C.; Ait Barka, E.; Kerzaon, I.; Sanchez, L. Impact of Paraburkholderia phytofirmans PsJN on Grapevine Phenolic Metabolism. Int. J. Mol. Sci. 2019, 20, 5775.
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