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Open AccessArticle

Sequential Transcriptome Changes in the Penumbra after Ischemic Stroke

1
Center for Neuroscience Research, Institute of Biomedical Science and Technology, Konkuk University, Seoul 05029, Korea
2
Department of Medical Science Konkuk University School of Medicine, Konkuk University, Seoul 05029, Korea
3
Department of Rehabilitation Medicine, Konkuk University School of Medicine, Konkuk University, Seoul 05029, Korea
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(24), 6349; https://doi.org/10.3390/ijms20246349
Received: 13 November 2019 / Revised: 11 December 2019 / Accepted: 14 December 2019 / Published: 16 December 2019
(This article belongs to the Special Issue Molecular Research on Neurodegenerative Diseases 2.0)
To investigate the changes in the expression of specific genes that occur during the acute-to-chronic post-stroke phase, we identified differentially expressed genes (DEGs) between naive cortical tissues and peri-infarct tissues at 1, 4, and 8 weeks after photothrombotic stroke. The profiles of DEGs were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and gene ontology analyses, followed by string analysis of the protein–protein interactions (PPI) of the products of these genes. We found 3771, 536, and 533 DEGs at 1, 4, and 8 weeks after stroke, respectively. A marked decrease in biological–process categories, such as brain development and memory, and a decrease in neurotransmitter synaptic and signaling pathways were observed 1 week after stroke. The PPI analysis showed the downregulation of Dlg4, Bdnf, Gria1, Rhoa, Mapk8, and glutamatergic receptors. An increase in biological–process categories, including cell population proliferation, cell adhesion, and inflammatory responses, was detected at 4 and 8 weeks post-stroke. The KEGG pathways of complement and coagulation cascades, phagosomes, antigen processing, and antigen presentation were also altered. CD44, C1, Fcgr2b, Spp1, and Cd74 occupied a prominent position in network analyses. These time-dependent changes in gene profiles reveal the unique pathophysiological characteristics of stroke and suggest new therapeutic targets for this disease. View Full-Text
Keywords: stroke; ischemic penumbra; transcriptome; gene profile alterations; acute stroke; chronic stroke stroke; ischemic penumbra; transcriptome; gene profile alterations; acute stroke; chronic stroke
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Choi, I.-A.; Yun, J.H.; Kim, J.-H.; Kim, H.Y.; Choi, D.-H.; Lee, J. Sequential Transcriptome Changes in the Penumbra after Ischemic Stroke. Int. J. Mol. Sci. 2019, 20, 6349.

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