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Open AccessArticle

Identification of B Cell Epitopes of Blo t 13 Allergen and Cross-Reactivity with Human Adipocytes and Heart Fatty Acid Binding Proteins

1
Institute for Immunological Research, University of Cartagena, Cartagena 130000, Colombia
2
Centro Nacional de Biopreparados (BIOCEN), Bejucal 32600, Cuba
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(24), 6107; https://doi.org/10.3390/ijms20246107
Received: 21 October 2019 / Revised: 29 November 2019 / Accepted: 29 November 2019 / Published: 4 December 2019
(This article belongs to the Section Molecular Immunology)
Cross-reactivity between allergens and human proteins could have a clinical impact in allergic diseases. Blo t 13 is an allergen from the mite Blomia tropicalis, which belongs to the fatty acid binding protein (FABP) family and has structural homology with human FABPs. This work aimed to map B cell epitopes on Blo t 13 and to identify epitopes involved in cross-reactivity with human heart FABP (FABP3) and adipocyte FABP (FABP4). Sera from 25 patients with house dust mite (HDM) allergy that were sensitized to Blo t 13 were used for testing the reactivity of immunoglobulin E (IgE) and IgG to FABP. The epitope mapping of Blo t 13 was performed using overlapping peptides, and cross-reactivity between Blo t 13 and human FABP was analyzed using human sera and anti-Blo t 13 monoclonal antibodies. IgE antibodies to all FABPs were detected in 14/25 serum samples, and IgG was detected in 25/25 serum samples. The cross-reactivity of Blo t 13 was 42% with FABP3 and 48% with FABP4. Two IgE-binding regions were identified in Blo t 13; one between residues 54 and 72 (the main cross-reacting region) and another between residues 111 to 129. Our results suggest that exposure to the Blo t 13 allergen could induce an auto-reactive response to endogenous FABP in allergic patients sensitized to Blo t 13. View Full-Text
Keywords: FABP; IgE; IgG; house dust mites; cross-reactivity; Blomia tropicalis FABP; IgE; IgG; house dust mites; cross-reactivity; Blomia tropicalis
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Múnera, M.; Martínez, D.; Labrada, A.; Caraballo, L.; Puerta, L. Identification of B Cell Epitopes of Blo t 13 Allergen and Cross-Reactivity with Human Adipocytes and Heart Fatty Acid Binding Proteins. Int. J. Mol. Sci. 2019, 20, 6107.

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