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Article

Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components

1
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
2
Weill Cornell Medical College, Cornell University, New York, NY 10065, USA
3
Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(23), 5839; https://doi.org/10.3390/ijms20235839
Received: 24 September 2019 / Revised: 5 November 2019 / Accepted: 7 November 2019 / Published: 20 November 2019
(This article belongs to the Special Issue Nuclear Envelope Proteins)
The nuclei of cells may exhibit invaginations of the nuclear envelope under a variety of conditions. These invaginations form a branched network termed the nucleoplasmic reticulum (NR), which may be found in cells in pathological and physiological conditions. While an extensive NR is a hallmark of cellular senescence and shows associations with some cancers, very little is known about the formation of NR in physiological conditions, despite the presence of extensive nuclear invaginations in some cell types such as endometrial cells. Here we show that in these cells the NR is formed in response to reproductive hormones. We demonstrate that oestrogen and progesterone are sufficient to induce NR formation and that this process is reversible without cell division upon removal of the hormonal stimulus. Nascent lamins and phospholipids are incorporated into the invaginations suggesting that there is a dedicated machinery for its formation. The induction of NR in endometrial cells offers a new model to study NR formation and function in physiological conditions. View Full-Text
Keywords: nuclear architecture; nucleoplasmic reticulum; reproductive cycle nuclear architecture; nucleoplasmic reticulum; reproductive cycle
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MDPI and ACS Style

Pytowski, L.; Drozdz, M.M.; Jiang, H.; Hernandez, Z.; Kumar, K.; Knott, E.; Vaux, D.J. Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components. Int. J. Mol. Sci. 2019, 20, 5839. https://doi.org/10.3390/ijms20235839

AMA Style

Pytowski L, Drozdz MM, Jiang H, Hernandez Z, Kumar K, Knott E, Vaux DJ. Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components. International Journal of Molecular Sciences. 2019; 20(23):5839. https://doi.org/10.3390/ijms20235839

Chicago/Turabian Style

Pytowski, Lior, Marek M. Drozdz, Haibo Jiang, Zayra Hernandez, Kurun Kumar, Emily Knott, and David J. Vaux. 2019. "Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components" International Journal of Molecular Sciences 20, no. 23: 5839. https://doi.org/10.3390/ijms20235839

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