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Open AccessArticle

SH3Ps—Evolution and Diversity of a Family of Proteins Engaged in Plant Cytokinesis

Department of Experimental Plant Biology, Faculty of Sciences, Charles University, Viničná 5, CZ 128 43 Prague, Czech Republic
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(22), 5623; https://doi.org/10.3390/ijms20225623
Received: 24 October 2019 / Revised: 4 November 2019 / Accepted: 6 November 2019 / Published: 11 November 2019
(This article belongs to the Section Molecular Plant Sciences)
SH3P2 (At4g34660), an Arabidopsis thaliana SH3 and Bin/amphiphysin/Rvs (BAR) domain-containing protein, was reported to have a specific role in cell plate assembly, unlike its paralogs SH3P1 (At1g31440) and SH3P3 (At4g18060). SH3P family members were also predicted to interact with formins—evolutionarily conserved actin nucleators that participate in microtubule organization and in membrane–cytoskeleton interactions. To trace the origin of functional specialization of plant SH3Ps, we performed phylogenetic analysis of SH3P sequences from selected plant lineages. SH3Ps are present in charophytes, liverworts, mosses, lycophytes, gymnosperms, and angiosperms, but not in volvocal algae, suggesting association of these proteins with phragmoplast-, but not phycoplast-based cell division. Separation of three SH3P clades, represented by SH3P1, SH3P2, and SH3P3 of A. thaliana, appears to be a seed plant synapomorphy. In the yeast two hybrid system, Arabidopsis SH3P3, but not SH3P2, binds the FH1 and FH2 domains of the formin FH5 (At5g54650), known to participate in cytokinesis, while an opposite binding specificity was found for the dynamin homolog DRP1A (At5g42080), confirming earlier findings. This suggests that the cytokinetic role of SH3P2 is not due to its interaction with FH5. Possible determinants of interaction specificity of SH3P2 and SH3P3 were identified bioinformatically. View Full-Text
Keywords: cytokinesis; evolution; phylogeny; cell plate; formin; interaction specificity cytokinesis; evolution; phylogeny; cell plate; formin; interaction specificity
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Baquero Forero, A.; Cvrčková, F. SH3Ps—Evolution and Diversity of a Family of Proteins Engaged in Plant Cytokinesis. Int. J. Mol. Sci. 2019, 20, 5623.

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