Water Extract of Cryphaea heteromalla (Hedw.) D. Mohr Bryophyte as a Natural Powerful Source of Biologically Active Compounds

Provenzano, Fiorenza; Sánchez, Jesús Lozano; Rao, Estella; Santonocito, Radha; Ditta, Lorena Anna; Borrás Linares, Isabel; Passantino, Rosa; Campisi, Patrizia; Dia, Maria Giovanna; Costa, Maria Assunta; Segura-Carretero, Antonio; San Biagio, Pier Luigi; Giacomazza, Daniela

doi:10.3390/ijms20225560

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Water Extract of Cryphaea heteromalla (Hedw.) D. Mohr Bryophyte as a Natural Powerful Source of Biologically Active Compounds

^1,†,

^2,3,†

¹,

¹,

¹,

Isabel Borrás Linares

³,

⁴,

⁴,

^1,*

Antonio Segura-Carretero

^3,5,

Pier Luigi San Biagio

^1,*

and

Daniela Giacomazza

Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Via U. La Malfa, 153, 90146 Palermo, Italy

Department of Food Science and Nutrition, University of Granada, Campus Universitario s/n, 18071 Granada, Spain

Center of Research and Development of Functional Food, Health Science Technological Park, Avda. del Conocimiento s/n, 18100 Granada, Spain

⁴

Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Università di Palermo, Via Archirafi 38, 90123 Palermo, Italy

⁵

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, Spain

Authors to whom correspondence should be addressed.

^†

These Authors contributed equally to the work.

Int. J. Mol. Sci. 2019, 20(22), 5560; https://doi.org/10.3390/ijms20225560

Received: 8 October 2019 / Revised: 29 October 2019 / Accepted: 31 October 2019 / Published: 7 November 2019

(This article belongs to the Section Bioactives and Nutraceuticals)

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Abstract

Bryophytes comprise of the mosses, liverworts, and hornworts. Cryphaea heteromalla, (Hedw.) D. Mohr, is a non-vascular lower plant belonging to mosses group. To the date, the most chemically characterized species belong to the liverworts, while only 3.2% and 8.8% of the species belonging to the mosses and hornworts, respectively, have been investigated. In this work, we present Folin–Ciocalteu and oxygen radical absorbance capacity (ORAC) data related to crude extracts of C. heteromalla obtained by three different extraction solvents: pure water (WT), methanol:water (80:20 v/v) (MET), and ethanol:water (80:20 v/v) (ETH). The water extract proved to be the best solvent showing the highest content of biophenols and the highest ORAC value. The C. heteromalla-WT extract was investigated by HPLC-TOF/MS (High Performance Liquid Chromatography-Time of Flight/Mass Spectrometry) allowing for the detection of 14 compounds, five of which were phenolic compounds, derivatives of benzoic, caffeic, and coumaric acids. Moreover, the C. heteromalla WT extract showed a protective effect against reactive oxygen species (ROS) generation induced by tert-butyl hydroperoxide (TBH) on the murine NIH-3T3 fibroblast cell line. View Full-Text

Keywords: bryophytes; Cryphaea heteromalla; extract HPLC-ESI-TOF-MS analysis; oxidative stress evaluation

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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MDPI and ACS Style

Provenzano, F.; Sánchez, J.L.; Rao, E.; Santonocito, R.; Ditta, L.A.; Borrás Linares, I.; Passantino, R.; Campisi, P.; Dia, M.G.; Costa, M.A.; Segura-Carretero, A.; San Biagio, P.L.; Giacomazza, D. Water Extract of Cryphaea heteromalla (Hedw.) D. Mohr Bryophyte as a Natural Powerful Source of Biologically Active Compounds. Int. J. Mol. Sci. 2019, 20, 5560. https://doi.org/10.3390/ijms20225560

AMA Style

Provenzano F, Sánchez JL, Rao E, Santonocito R, Ditta LA, Borrás Linares I, Passantino R, Campisi P, Dia MG, Costa MA, Segura-Carretero A, San Biagio PL, Giacomazza D. Water Extract of Cryphaea heteromalla (Hedw.) D. Mohr Bryophyte as a Natural Powerful Source of Biologically Active Compounds. International Journal of Molecular Sciences. 2019; 20(22):5560. https://doi.org/10.3390/ijms20225560

Chicago/Turabian Style

Provenzano, Fiorenza, Jesús L. Sánchez, Estella Rao, Radha Santonocito, Lorena A. Ditta, Isabel Borrás Linares, Rosa Passantino, Patrizia Campisi, Maria G. Dia, Maria A. Costa, Antonio Segura-Carretero, Pier L. San Biagio, and Daniela Giacomazza. 2019. "Water Extract of Cryphaea heteromalla (Hedw.) D. Mohr Bryophyte as a Natural Powerful Source of Biologically Active Compounds" International Journal of Molecular Sciences 20, no. 22: 5560. https://doi.org/10.3390/ijms20225560

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Water Extract of Cryphaea heteromalla (Hedw.) D. Mohr Bryophyte as a Natural Powerful Source of Biologically Active Compounds

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