Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin
Abstract
:1. Introduction
2. Results
2.1. MMP-3 Expression in the Skin of the Four Murine Genotypes
2.2. Collagen Degradation in Incubated Skin Explants of the Four Murine Genotypes
2.3. MMP-13 in MMP-3-Deficient and MMP-3-Overexpressing Conditions
2.4. Effect of Pro-Inflammatory TNF-α on MMP-3 Tissue Levels, MMP-13 Secretion and Collagen Degradation
2.5. Effect of Pro-Inflammatory TNF-α on MMP-2 Secretion in MMP-3-Deficient and -Overexpressing Conditions
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Experimental Model and Treatment Groups
4.3. Analyses
4.3.1. Hydroxyproline Assay
4.3.2. MMP-3 and MMP-13 mRNA Determined by qPCR
4.3.3. Casein and Gelatin Zymography
4.3.4. Collagenase Activity
4.3.5. Western Blot Analyses
4.4. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
APMA | Aminophenylmercuric acetate |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase |
IL-1β | Interleukin-1β |
LRP | Lipoprotein receptor-related protein |
MMP | Matrix metalloproteinase |
PVDF | Polyvinylidene fluoride |
RAP | Receptor-associated protein |
TIMP | Tissue inhibitor of metalloproteinases |
TNF-α | Tumor necrosis factor-α |
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Day | KO (n = 10) | WT (n = 10) | WTT (n = 9) | TG (n = 10) |
---|---|---|---|---|
0 | 0 | 0 | 0 | 0 |
2 | 3.9 ± 0.2 | 4.3 ± 0.5 | 6.8 ± 1.7 | 5.0 ± 1.0 |
4 | 29.9 ± 3.5 | 49.3 ± 4.7 | 94.3 ± 12.0 | 74.4 ± 10.5 |
6 | 32.4 ± 3.9 | 74.9 ± 8.4 | 79.0 ± 5.7 | 92.4 ± 11.2 |
8 | 24.1 ± 3.6 | 59.0 ± 5.5 | 124.4 ± 16.7 | 113.2 ± 11.8 |
Accumulated | 90.3 | 187.5 | 304.5 | 285.0 |
Regression analysis | ||||
Slope | 3.16 | 9.49 | 16.9 | 17.1 |
p | <0.001 | 0.47 |
Group | Control | TNF-α 1 | GM6001 2 |
---|---|---|---|
KO | n = 10 | n = 10 | n = 5 |
WT | n = 10 | n = 10 | n = 5 |
WTT | n = 9 | n = 9 | n = 4 |
TG | n = 10 | n = 10 | n = 5 |
Group | Control | TNF-α 1 | GM6001 2 | |||
---|---|---|---|---|---|---|
0 | +RAP 3 | 0 | +RAP 3 | 0 | +RAP 3 | |
KO | n = 4 | n = 4 | n = 4 | n = 4 | n = 4 | n = 4 |
WT | n = 4 | n = 4 | n = 4 | n = 4 | n = 4 | n = 4 |
WTT | n = 4 | n = 4 | n = 4 | n = 4 | n = 4 | n = 4 |
TG | n = 4 | n = 4 | n = 4 | n = 4 | n = 4 | n = 4 |
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Mirastschijski, U.; Lupše, B.; Maedler, K.; Sarma, B.; Radtke, A.; Belge, G.; Dorsch, M.; Wedekind, D.; McCawley, L.J.; Boehm, G.; et al. Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin. Int. J. Mol. Sci. 2019, 20, 5234. https://doi.org/10.3390/ijms20205234
Mirastschijski U, Lupše B, Maedler K, Sarma B, Radtke A, Belge G, Dorsch M, Wedekind D, McCawley LJ, Boehm G, et al. Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin. International Journal of Molecular Sciences. 2019; 20(20):5234. https://doi.org/10.3390/ijms20205234
Chicago/Turabian StyleMirastschijski, Ursula, Blaž Lupše, Kathrin Maedler, Bhavishya Sarma, Arlo Radtke, Gazanfer Belge, Martina Dorsch, Dirk Wedekind, Lisa J. McCawley, Gabriele Boehm, and et al. 2019. "Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin" International Journal of Molecular Sciences 20, no. 20: 5234. https://doi.org/10.3390/ijms20205234