MicroRNA Mediate Visfatin and Resistin Induction of Oxidative Stress in Human Osteoarthritic Synovial Fibroblasts Via NF-κB Pathway
Abstract
:1. Introduction
2. Results
2.1. Cell viability Evaluation in Visfatin and Resistin Treated Cells
2.2. Visfatin and Resistin Promote Inflammation and Regulate Cartilage Turnover
2.3. Adipokines Induce Apoptosis and Regulate BCL2 Expression
2.4. Visfatin and Resistin Regulate Oxidant/Antioxidant Balance
2.5. Visfatin and Resistin Modulate miRNA Gene Expression
2.6. MiRNA Regulate Oxidative Stress Induced by Visfatin and Resistin
2.7. Visfatin and Resistin Activate NF-κB Signaling Pathway
2.8. NF-κB Signaling Pathway Inhibits Visfatin and Resistin Effects
3. Discussion
4. Materials and Methods
4.1. Sample Collection and Cell Culture
4.2. Stimulus of Synovial Cell Cultures
4.3. MTT Assay
4.4. Transfection of Synovial Cells
4.5. Quantitative Real-Time PCR of mRNA and miRNA
4.6. Apoptosis Detection
4.7. Mitochondrial Superoxide Anion (•O2-) Production
4.8. Immunofluorescence Analysis
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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miRNA Genes | Cat. No. (Qiagen) |
miR-34a | MS00003318 |
miR-146a | MS00003535 |
miR-181a | MS00006692 |
SNORD-25 | MS00014007 |
Target Genes | Cat. No. (Qiagen) |
IL-1β | QT00021385 |
IL-6 | QT00083720 |
IL-17A | QT00009233 |
TNF-α | QT00029162 |
MMP-1 | QT00014581 |
MMP-13 | QT00001764 |
Col2a1 | QT00049518 |
BCL2 | QT00000721 |
SOD-2 | QT01008693 |
CAT | QT00079674 |
NRF2 | QT00027384 |
ACTB | QT00095431 |
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Cheleschi, S.; Gallo, I.; Barbarino, M.; Giannotti, S.; Mondanelli, N.; Giordano, A.; Tenti, S.; Fioravanti, A. MicroRNA Mediate Visfatin and Resistin Induction of Oxidative Stress in Human Osteoarthritic Synovial Fibroblasts Via NF-κB Pathway. Int. J. Mol. Sci. 2019, 20, 5200. https://doi.org/10.3390/ijms20205200
Cheleschi S, Gallo I, Barbarino M, Giannotti S, Mondanelli N, Giordano A, Tenti S, Fioravanti A. MicroRNA Mediate Visfatin and Resistin Induction of Oxidative Stress in Human Osteoarthritic Synovial Fibroblasts Via NF-κB Pathway. International Journal of Molecular Sciences. 2019; 20(20):5200. https://doi.org/10.3390/ijms20205200
Chicago/Turabian StyleCheleschi, Sara, Ines Gallo, Marcella Barbarino, Stefano Giannotti, Nicola Mondanelli, Antonio Giordano, Sara Tenti, and Antonella Fioravanti. 2019. "MicroRNA Mediate Visfatin and Resistin Induction of Oxidative Stress in Human Osteoarthritic Synovial Fibroblasts Via NF-κB Pathway" International Journal of Molecular Sciences 20, no. 20: 5200. https://doi.org/10.3390/ijms20205200