Pharmacological Enhancement of Regeneration-Dependent Regulatory T Cell Recruitment in Zebrafish
Abstract
:1. Introduction
2. Results
2.1. Chemical Screen for Modulators of zTreg Recruitment to Regenerating Tail Tissue
2.2. Pramipexole Stimulates zTreg Recruitment in an Injury-Dependent Manner
2.3. Pramipexole Stimulates zTreg Recruitment via Dopaminergic Pathways
3. Discussion
4. Materials and Methods
4.1. Zebrafish Maintenance and Breeding
4.2. Zebrafish Transgenic Lines
4.3. Juvenile Fin Amputation
4.4. Chemical Screen and Drug Treatments
4.5. zTreg Quantification
4.6. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AMI | amisulpride |
APO | apomorphine |
BAC | Bacterial Artificial Chromosome |
DMSO | dimethyl sulfoxide |
dpi | days post-injury |
EGFP | Enhanced GFP |
FOXP3 | Forkhead Box P3 |
PD | Parkinson’s Disease |
PPX | pramipexole |
RFP | Red Fluorescent Protein |
SCH | SCH-23390 |
Treg | regulatory T cell |
wpf | weeks post-fertilization |
zTreg | zebrafish regulatory T cell |
Appendix A
Drug Type 1 | Drug Class | Identity |
---|---|---|
Immune | Glucocorticoid | CPD000058331 |
suppressants | Glucocorticoid | CPD000058335 |
Glucocorticoid | Amcinonide | |
Glucocorticoid | Beclomethasone | |
Glucocorticoid | Clobetasol proprionate | |
Glucocorticoid | Fluorometholone | |
Glucocorticoid | Fluticasone proprionate | |
Glucocorticoid | Halometasone monohydrate | |
Glucocorticoid | Westcort | |
Glucocorticoid | Beclomethasone dipropionate | |
Glucocorticoid | Prednisolone acetate | |
Glucocorticoid | (R)-Budesonide | |
Glucocorticoid | Loteprednol Etabonate | |
IL-2 immunosuppressant | Tacrolimus | |
CNS agents | Phenothiazine | Thioridazine hydrochloride |
Phenothiazine | Chlorpromazine hydrochloride | |
Tricyclic antidepressant | Amoxapine | |
Tricyclic antidepressant | Maprotilline HCl | |
Tricyclic antidepressant | Desipramine hydrochloride | |
Selective serotonin reuptake inhibitor | Paroxetine | |
Serotonin receptor antagonist | Tropisetron hydrochloride | |
Cyclopyrrolone | Eszopiclone | |
Benzodiazepine | Chlordiazepoxide | |
Opioid antagonist | Naloxone hydrochloride | |
Nicotine receptor agonist | Nicotine | |
Anaesthetic | Procaine hydrochloride | |
Antimicrobial | Antibiotic | Doxycycline |
Antibiotic | Sulfisoxazole | |
Antiparasitic | Thiabendazole | |
Antiparasitic | Mebendazole | |
Antifungal | Griseofulvin | |
Antiviral | Ganciclovir | |
Diuretic | Diuretic | Bendrofluazide |
Carbonic anhydrase inhibitor | Methazolamide | |
Sodium channel blocker | Triamterene | |
Hypoglycemic | Enzyme inhibitor | Etomoxir |
Potassium channel blocker | Tolbutamide | |
Antineoplastic | Antineoplastic | 6-Azauridine |
Antineoplastic | Mitoxantrone | |
Other | PDE5 inhibitor | Tadalafil |
Adrenoreceptor agonist | Brimonidine | |
Uricosuric | Sulfinpyrazone | |
Anticoagulant | Phylloquinone | |
Antiarrhythmic | Pronestyl |
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Zwi, S.F.; Choron, C.; Zheng, D.; Nguyen, D.; Zhang, Y.; Roshal, C.; Kikuchi, K.; Hesselson, D. Pharmacological Enhancement of Regeneration-Dependent Regulatory T Cell Recruitment in Zebrafish. Int. J. Mol. Sci. 2019, 20, 5189. https://doi.org/10.3390/ijms20205189
Zwi SF, Choron C, Zheng D, Nguyen D, Zhang Y, Roshal C, Kikuchi K, Hesselson D. Pharmacological Enhancement of Regeneration-Dependent Regulatory T Cell Recruitment in Zebrafish. International Journal of Molecular Sciences. 2019; 20(20):5189. https://doi.org/10.3390/ijms20205189
Chicago/Turabian StyleZwi, Stephanie F., Clarisse Choron, Dawei Zheng, David Nguyen, Yuxi Zhang, Camilla Roshal, Kazu Kikuchi, and Daniel Hesselson. 2019. "Pharmacological Enhancement of Regeneration-Dependent Regulatory T Cell Recruitment in Zebrafish" International Journal of Molecular Sciences 20, no. 20: 5189. https://doi.org/10.3390/ijms20205189