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Open AccessArticle

Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice

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Faculty of Medicine Osijek, University Josip Juraj Strossmayer Osijek, Institute and Dept of Physiology and Immunology, J. Huttlera 4, HR-31000 Osijek, Croatia
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Ruđer Bošković Institute, Department of Molecular Medicine; Bijenička 54, HR-10000 Zagreb, Croatia
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Clinical Hospital Center Osijek, Dept of Dermatology and Venerology, J. Huttlera 4, HR-31000 Osijek, Croatia
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Clinical Hospital Center Osijek, Neurology Clinic, J. Huttlera 4, HR-31000 Osijek, Croatia
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University of Zagreb, School of Medicine, Croatian Institute for Brain Research, Zagreb, Croatia, Šalata 12, HR-10000 Zagreb, Croatia
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Proteomics laboratory, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55 HR-10000 Zagreb, Croatia
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Faculty of Medicine Osijek, University Josip Juraj Strossmayer Osijek, Institute and Dept of Histology and Embriology, J. Huttlera 4, HR-31000 Osijek, Croatia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(20), 5188; https://doi.org/10.3390/ijms20205188
Received: 29 July 2019 / Revised: 20 September 2019 / Accepted: 5 October 2019 / Published: 19 October 2019
(This article belongs to the Special Issue TFF Peptides: Lectins in Mucosal Protection and More)
High salt (HS) dietary intake leads to impaired vascular endothelium-dependent responses to various physiological stimuli, some of which are mediated by arachidonic acid (AA) metabolites. Transgenic Tff3−/− gene knockout mice (Tff3−/−/C57BL/6N) have changes in lipid metabolism which may affect vascular function and outcomes of stroke. We aimed to study the effects of one week of HS diet (4% NaCl) on vascular function and stroke induced by transient occlusion of middle cerebral artery in Tff3−/− and wild type (WT/C57BL/6N) mice. Flow-induced dilation (FID) of carotid artery was reduced in WT-HS mice, but not affected in Tff3−/−-HS mice. Nitric oxide (NO) mediated FID. NO production was decreased with HS diet. On the contrary, acetylcholine-induced dilation was significantly decreased in Tff3−/− mice on both diets and WT-HS mice. HS intake and Tff3 gene depletion affected the structural components of the vessels. Proteomic analysis revealed a significant effect of Tff3 gene deficiency on HS diet-induced changes in neuronal structural proteins and acute innate immune response proteins’ expression and Tff3 depletion, but HS diet did not increase the stroke volume, which is related to proteome modification and upregulation of genes involved mainly in cellular antioxidative defense. In conclusion, Tff3 depletion seems to partially impair vascular function and worsen the outcomes of stroke, which is moderately affected by HS diet. View Full-Text
Keywords: Tff3 gene; flow-induced dilation; high salt diet; stroke Tff3 gene; flow-induced dilation; high salt diet; stroke
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Kozina, N.; Mihaljević, Z.; Baus Lončar, M.; Mihalj, M.; Mišir, M.; Radmilović, M.D.; Justić, H.; Gajović, S.; Šešelja, K.; Bazina, I.; Horvatić, A.; Matić, A.; Bijelić, N.; Rođak, E.; Jukić, I.; Drenjančević, I. Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice. Int. J. Mol. Sci. 2019, 20, 5188.

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