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Article

Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding Proteins

Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain
*
Author to whom correspondence should be addressed.
Present Address: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus de Cartuja s/n, 18071 Granada, Spain.
Present Address: Department of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia, 30100 Murcia, Spain.
§
Present Address: Department of Biochemistry and Molecular Biology II, Pharmacy School, Granada University, 18011 Granada, Spain.
Int. J. Mol. Sci. 2019, 20(20), 5156; https://doi.org/10.3390/ijms20205156
Received: 17 September 2019 / Revised: 15 October 2019 / Accepted: 16 October 2019 / Published: 17 October 2019
(This article belongs to the Special Issue Bacterial Chemoreceptors)
Solute binding proteins (SBPs) form a heterogeneous protein family that is found in all kingdoms of life. In bacteria, the ligand-loaded forms bind to transmembrane transporters providing the substrate. We present here the SBP repertoire of Pseudomonas aeruginosa PAO1 that is composed of 98 proteins. Bioinformatic predictions indicate that many of these proteins have a redundant ligand profile such as 27 SBPs for proteinogenic amino acids, 13 proteins for spermidine/putrescine, or 9 proteins for quaternary amines. To assess the precision of these bioinformatic predictions, we have purified 17 SBPs that were subsequently submitted to high-throughput ligand screening approaches followed by isothermal titration calorimetry studies, resulting in the identification of ligands for 15 of them. Experimentation revealed that PA0222 was specific for γ-aminobutyrate (GABA), DppA2 for tripeptides, DppA3 for dipeptides, CysP for thiosulphate, OpuCC for betaine, and AotJ for arginine. Furthermore, RbsB bound D-ribose and D-allose, ModA bound molybdate, tungstate, and chromate, whereas AatJ recognized aspartate and glutamate. The majority of experimentally identified ligands were found to be chemoattractants. Data show that the ligand class recognized by SPBs can be predicted with confidence using bioinformatic methods, but experimental work is necessary to identify the precise ligand profile. View Full-Text
Keywords: solute binding protein; transport; chemotaxis; ligand recognition solute binding protein; transport; chemotaxis; ligand recognition
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MDPI and ACS Style

Fernández, M.; Rico-Jiménez, M.; Ortega, Á.; Daddaoua, A.; García García, A.I.; Martín-Mora, D.; Mesa Torres, N.; Tajuelo, A.; Matilla, M.A.; Krell, T. Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding Proteins. Int. J. Mol. Sci. 2019, 20, 5156. https://doi.org/10.3390/ijms20205156

AMA Style

Fernández M, Rico-Jiménez M, Ortega Á, Daddaoua A, García García AI, Martín-Mora D, Mesa Torres N, Tajuelo A, Matilla MA, Krell T. Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding Proteins. International Journal of Molecular Sciences. 2019; 20(20):5156. https://doi.org/10.3390/ijms20205156

Chicago/Turabian Style

Fernández, Matilde, Miriam Rico-Jiménez, Álvaro Ortega, Abdelali Daddaoua, Ana I. García García, David Martín-Mora, Noel Mesa Torres, Ana Tajuelo, Miguel A. Matilla, and Tino Krell. 2019. "Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding Proteins" International Journal of Molecular Sciences 20, no. 20: 5156. https://doi.org/10.3390/ijms20205156

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