HDAC2 Regulates Glial Cell Activation in Ischemic Mouse Retina
Abstract
:1. Introduction
2. Results
2.1. Expression of GFAP, Iba1, HIF-1α, and Acetyl-H3 in Retinas after IR Injury
2.2. Effect of TSA on Expression of GFAP, Iba1, HIF-1α, and Acetyl-H3 in Retinas after IR Injury
2.3. Effect of Overexpression of HDAC1 and HDAC2 on Glial Cell Activity and iNOS Expression
2.4. Effect of TSA on RGC Loss in Retina after IR Injury
3. Discussion
4. Materials and Methods
4.1. Animal Use
4.2. Retinal Ischemia-Reperfusion (IR) Injury
4.3. Adenoviral GFP, HDAC1, and HDAC2
4.4. Real-Time Polymerase Chain Reaction
4.5. Pharmacological Treatment
4.6. Western Blot Analysis
4.7. Immunohistochemical Analysis
4.8. Retinal Wholemounts and Brn3a Staining
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Cell Density (cell/mm2 ± SD) | |||
---|---|---|---|
Control | Saline-Treated IR Injury Group | TSA-Treated IR Injury Group | |
Center | 1920 ± 289.20 | 1576 ± 277.69 * | 1779± 234.25 *† |
Middle | 1714 ± 259.69 | 1393 ± 334.10 * | 1608 ± 299.62 *† |
Peripheral | 1723 ± 259.75 | 1320 ± 299.96 * | 1501 ± 284.41 *† |
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Sung, M.S.; Heo, H.; Eom, G.H.; Kim, S.Y.; Piao, H.; Guo, Y.; Park, S.W. HDAC2 Regulates Glial Cell Activation in Ischemic Mouse Retina. Int. J. Mol. Sci. 2019, 20, 5159. https://doi.org/10.3390/ijms20205159
Sung MS, Heo H, Eom GH, Kim SY, Piao H, Guo Y, Park SW. HDAC2 Regulates Glial Cell Activation in Ischemic Mouse Retina. International Journal of Molecular Sciences. 2019; 20(20):5159. https://doi.org/10.3390/ijms20205159
Chicago/Turabian StyleSung, Mi Sun, Hwan Heo, Gwang Hyeon Eom, So Young Kim, Helong Piao, Yue Guo, and Sang Woo Park. 2019. "HDAC2 Regulates Glial Cell Activation in Ischemic Mouse Retina" International Journal of Molecular Sciences 20, no. 20: 5159. https://doi.org/10.3390/ijms20205159