Osteogenic Differentiation of Mesenchymal Stem Cells with Silica-Coated Gold Nanoparticles for Bone Tissue Engineering
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Nanofibrous Scaffolds
2.2. Surface Wettability
2.3. Porosity
2.4. Mechanical Strength
2.5. Cell Proliferation
2.6. Cell-Scaffold Interactions
2.7. CMFDA (5-Chloromethylfluorescein Diacetate) Dye Assay
2.8. Alkaline Phosphatase (ALP) Activity
2.9. Alizarin Red S (ARS) Staining
2.10. Expression of Osteocalcin (OCN)
3. Materials and Methods
3.1. Materials
3.2. Fabrication of Nanofibrous Scaffolds
3.3. Characterization of Nanofibrous Scaffolds
3.4. In-vitro Culture of Human Mesenchymal Stem Cells (hMSCs)
3.5. Cell Proliferation Assay
3.6. CMFDA Staining
3.7. ALP Activity
3.8. ARS Staining
3.9. Immunofluorescence Staining
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Nanofibrous Constructs | Fiber Diameter (nm) | Pore Size (μm) | Porosity (%) | Tensile Strength (MPa) |
---|---|---|---|---|
PCL | 215 ± 32.12 | 1.45 ± 0.26 | 88 ± 4.3 | 7.63 |
PCL/SF | 164 ± 18.65 | 2.12 ± 0.31 | 92 ± 6.3 | 11.67 |
PCL/SF/Au(SiO2) | 172 ± 24.22 | 2.35 ± 0.22 | 90 ± 7.5 | 12.11 |
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Gandhimathi, C.; Quek, Y.J.; Ezhilarasu, H.; Ramakrishna, S.; Bay, B.-H.; Srinivasan, D.K. Osteogenic Differentiation of Mesenchymal Stem Cells with Silica-Coated Gold Nanoparticles for Bone Tissue Engineering. Int. J. Mol. Sci. 2019, 20, 5135. https://doi.org/10.3390/ijms20205135
Gandhimathi C, Quek YJ, Ezhilarasu H, Ramakrishna S, Bay B-H, Srinivasan DK. Osteogenic Differentiation of Mesenchymal Stem Cells with Silica-Coated Gold Nanoparticles for Bone Tissue Engineering. International Journal of Molecular Sciences. 2019; 20(20):5135. https://doi.org/10.3390/ijms20205135
Chicago/Turabian StyleGandhimathi, Chinnasamy, Ying Jie Quek, Hariharan Ezhilarasu, Seeram Ramakrishna, Boon-Huat Bay, and Dinesh Kumar Srinivasan. 2019. "Osteogenic Differentiation of Mesenchymal Stem Cells with Silica-Coated Gold Nanoparticles for Bone Tissue Engineering" International Journal of Molecular Sciences 20, no. 20: 5135. https://doi.org/10.3390/ijms20205135